aspA:Gene Product(s): Difference between revisions

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touch the Product_motif_table table.
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{| id="I46ab1fe783fe7"  class=" tableEdit Product_nomenclature_table"   
{|   id="I46ab1fe783fe7"  class=" tableEdit Product_nomenclature_table"   


|-
|-
!align=left {{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
||
||
AspA product
AspA  
|-
|-
!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
aspartate ammonia-lyase product<ref name='LIB:Riley_2006'/>, B4139 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AspA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''aspartate ammonia-lyase<ref name='LIB:Riley_2006'/>, B4139<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AspA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK4133, JW4099, b4139''
|-
|-
!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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{| {{Prettytable}} id="W46ab1fe993888"  class=" tableEdit GO_table_product dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="W46ab1fe993888"  class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|
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catalytic activity
catalytic activity
|
|
GO_REF:0000002<br />GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR000362<br />InterPro:IPR008948
InterPro:IPR000362
|
|
F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0006531
GO:0003824
|
|
aspartate metabolic process
catalytic activity
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR004708
InterPro:IPR008948
|
|
P
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0008797
GO:0003824
|
|
aspartate ammonia-lyase activity
catalytic activity
|
|
GO_REF:0000002<br />GO_REF:0000003
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR004708<br />EC:4.3.1.1
InterPro:IPR020557
|
|
F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0016829
GO:0005829
|
|
lyase activity
cytosol
|
|
GO_REF:0000004
PMID:16858726
|
|
IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
|
|
SP_KW:KW-0456
 
|
|
F
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
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GO:0005515
GO:0006099
|
|
protein binding
tricarboxylic acid cycle
|
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PMID:15690043
GOA:interpro<br />GO_REF:0000002
|
|
IPI: Inferred from Physical Interaction
IEA: Inferred from Electronic Annotation
|
|
UniProtKB:P0A6Y8
InterPro:IPR018951
|
|
F
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
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GO:0005624
GO:0006531
|
|
membrane fraction
aspartate metabolic process
|
|
PMID:16858726
GOA:interpro<br />GO_REF:0000002
|
|
IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
|
|
 
InterPro:IPR004708
|
|
C
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
|
GO:0005737
GO:0008797
|
|
cytoplasm
aspartate ammonia-lyase activity
|
|
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR004708
|
|
C
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-  
|-  
|
|


|
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GO:0005739
GO:0008797
|
|
mitochondrion
aspartate ammonia-lyase activity
|
|
PMID:16858726
GOA:spec<br />GO_REF:0000003
|
|
IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
|
|
 
EC:4.3.1.1
|
|
C
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0005829
GO:0016020
|
|
cytosol
membrane
|
|
PMID:16858726
PMID:16858726
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C
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
|
GO:0006520
GO:0016829
|
|
amino acid metabolic process
lyase activity
|
|
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR018951
|
|
P
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-  
|-  
|
|
 
|
|
GO:0006529
GO:0016829
|
|
asparagine biosynthetic process
lyase activity
|
|
GOA:spkw<br />SP_KW:GO_REF:0000004
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|
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0456
|
|
P
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-
|
|
GO:0009089
|
lysine biosynthetic process via diaminopimelate
|
|
|
|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing 


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46ab20084f4b6"  class=" tableEdit Product_interactions_table dataTable"   
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!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
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{|   id="Q46ab20099dde1"  class=" tableEdit Product_localization_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46ab20099dde1"  class=" tableEdit Product_localization_table"   
|- {{table heading style}}
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!|Compartment!!Description!!Evidence!!Source!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes


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!align=left {{table heading style}} |Name
||


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!align=left {{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
||
||
23,840 - 25,340 (calc based on 16 Y, W, and 12 C residues)
23,840 - 25,340 (calc based on 16 Y, 0 W, and 12 C residues)


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46ab200b4fba1"  class=" tableEdit Product_motif_table dataTable"   
|- {{table heading style}}
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!|Type!!Residues!!Description!!Notes!!References
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
13..345
|
[http://pfam.janelia.org/family/PF00206 PF00206  Lyase]
|
|
PMID:19920124
|-
|
Domain
|
411..465
|
[http://pfam.janelia.org/family/PF10415 PF10415  Fumarase C C-terminus]
|
|
PMID:19920124


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View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0AC38 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0AC38 PSI-PRED (P0AC38)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0AC38 Genomes TO Protein (P0AC38)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46ab200cb4cf0"  class=" tableEdit Product_resource_table"   
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!|Resource type!!Source!!Notes/Reference
!|Resource type!!Source!!Notes/Reference
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{|   id="Q46ab200d26c01"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46ab200d26c01"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=ASPARTASE-MONOMER EcoCyc:ASPARTASE-MONOMER]
[http://www.ncbi.nlm.nih.gov/protein/90111690 GI:90111690]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_418562.4 GeneID:948658]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0013551
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0AC38 UniProtKB/Swiss-Prot:P0AC38]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG10095
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG10095
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[[uniprot:P0AC38|UniProt:P0AC38]]
[http://www.ncbi.nlm.nih.gov/gene?term=948658 GeneID:948658]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://pfam.sanger.ac.uk/family?acc=PF00206 PFAM:PF00206]
RegulonDB:ECK120000091
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418562 RefSeq:NP_418562]
EchoBASE:EB0093
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0006520_!_amino acid metabolic process]][[Category:GO:0006529_!_asparagine biosynthetic process]][[Category:GO:0009089_!_lysine biosynthetic process via diaminopimelate]][[Category:Complex:aspartate ammonia-lyase]][[Category:Products with structures in PDB]]

Latest revision as of 21:13, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AspA

Synonyms

aspartate ammonia-lyase[1], B4139[2][1], AspA[2][1] , ECK4133, JW4099, b4139

Product description

AspA[2][3];

Component of aspartate ammonia-lyase[2][3]

L-Aspartate ammonia-lyase; L-aspartase[4]

EC number (for enzymes)
edit table


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000362

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008948

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020557

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006099

tricarboxylic acid cycle

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018951

P

Seeded from EcoCyc (v14.0)

complete

GO:0006531

aspartate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004708

P

Seeded from EcoCyc (v14.0)

complete

GO:0008797

aspartate ammonia-lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004708

F

Seeded from EcoCyc (v14.0)

complete

GO:0008797

aspartate ammonia-lyase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.3.1.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018951

F

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0456

F

Seeded from EcoCyc (v14.0)

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of aspartate ammonia-lyase

could be indirect

Protein

dnaK

PMID:15690043[6]

Experiment(s):EBI-878610, EBI-881328

Protein

secA

PMID:15690043[6]

Experiment(s):EBI-878610

Protein

otsB

PMID:16606699[7]

Experiment(s):EBI-1147527

Protein

chbF

PMID:16606699[7]

Experiment(s):EBI-1147527

Protein

nadE

PMID:16606699[7]

Experiment(s):EBI-1147527

Protein

rhsD

PMID:16606699[7]

Experiment(s):EBI-1147527

Protein

carB

PMID:16606699[7]

Experiment(s):EBI-1147527

Protein

yjbI

PMID:16606699[7]

Experiment(s):EBI-1147527
edit table


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
edit table


Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MCLKQIIGSL KKKVHMSNNI RIEEDLLGTR EVPADAYYGV HTLRAIENFY ISNNKISDIP
EFVRGMVMVK KAAAMANKEL QTIPKSVANA IIAACDEVLN NGKCMDQFPV DVYQGGAGTS
VNMNTNEVLA NIGLELMGHQ KGEYQYLNPN DHVNKCQSTN DAYPTGFRIA VYSSLIKLVD
AINQLREGFE RKAVEFQDIL KMGRTQLQDA VPMTLGQEFR AFSILLKEEV KNIQRTAELL
LEVNLGATAI GTGLNTPKEY SPLAVKKLAE VTGFPCVPAE DLIEATSDCG AYVMVHGALK
RLAVKMSKIC NDLRLLSSGP RAGLNEINLP ELQAGSSIMP AKVNPVVPEV VNQVCFKVIG
NDTTVTMAAE AGQLQLNVME PVIGQAMFES VHILTNACYN LLEKCINGIT ANKEVCEGYV
YNSIGIVTYL NPFIGHHNGD IVGKICAETG KSVREVVLER GLLTEAELDD IFSVQNLMHP
AYKAKRYTDE SEQ
Length

493

Mol. Wt

54.064 kDa

pI

5.6 (calculated)

Extinction coefficient

23,840 - 25,340 (calc based on 16 Y, 0 W, and 12 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

13..345

PF00206 Lyase

PMID:19920124[8]

Domain

411..465

PF10415 Fumarase C C-terminus

PMID:19920124[8]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aspA taxon=562,83333 </beststructure>

Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111690

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948658

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013551

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AC38

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10095

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10095

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948658

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000091

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0093

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ 6.0 6.1 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ 8.0 8.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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