recO:Gene Product(s): Difference between revisions

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|-
|-
!align=left |Standard name
!align=left {{table heading style}} |Standard name
||
||
RecO  
RecO  
|-
|-
!align=left |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
gap repair protein<ref name='LIB:Riley_2006'/>, B2565<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, RecO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''gap repair protein<ref name='LIB:Riley_2006'/>, B2565<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, RecO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2563, JW2549, b2565''
|-
|-
!align=left |Product description
!align=left {{table heading style}} |Product description
||
||
protein interacts with RecR and possibly RecF proteins<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
protein interacts with RecR and possibly RecF proteins<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
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Conjugational recombination and repair; DNA-binding protein; RecA-like strand assimilation<ref name='LIB:EcoGene'/>
Conjugational recombination and repair; DNA-binding protein; RecA-like strand assimilation<ref name='LIB:EcoGene'/>
|-
|-
!align=left |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
||
||


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{|   id="O46aab015e6df6"  class=" tableEdit GO_table_product dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="O46aab015e6df6"  class=" tableEdit GO_table_product dataTable"   
|-
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:hamap<br />GO_REF:0000020
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00201
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR003717
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0234
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-  
|-  
|
|


|
|
GO:0006974
GO:0006310
|
|
response to DNA damage stimulus
DNA recombination
|
|
GO_REF:0000004
GOA:hamap<br />GO_REF:0000020
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0227
HAMAP:MF_00201
|
|
P
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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DNA recombination
DNA recombination
|
|
GO_REF:0000002<br />GO_REF:0000004<br />GO_REF:0000020
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR003717<br />SP_KW:KW-0233<br />HAMAP:MF_00201
InterPro:IPR003717
|
|
P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0006281
GO:0006310
|
|
DNA repair
DNA recombination
|
|
GO_REF:0000002<br />GO_REF:0000004<br />GO_REF:0000020
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR003717<br />SP_KW:KW-0234<br />HAMAP:MF_00201
SP_KW:KW-0233
|
|
P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
|
GO:0005737
GO:0006974
|
|
cytoplasm
response to DNA damage stimulus
|
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0227
|
|
C
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete


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!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
|-  
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Experiment(s):EBI-1143287<br />  
Experiment(s):EBI-1143287<br />  
|-
|
Protein
|
rpsJ
|
|
PMID:19402753
|
LCMS(ID Probability):99.6


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!|Compartment!!Description!!Evidence!!Source!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes


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!align=left {{table heading style}} |Name
||


|-
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!align=left |Sequence
!align=left {{table heading style}} |Sequence
||
||
[http://biocyc.org/ECOLI/sequence-aa?object=EG10832-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=EG10832-MONOMER at EcoCyc]  
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KTFTGRQLKA LNAREFPDAD TLRAAKRFTR MALKPYLGGK PLKSRELFRQ FMPKRTVKTH YE</pre>  
KTFTGRQLKA LNAREFPDAD TLRAAKRFTR MALKPYLGGK PLKSRELFRQ FMPKRTVKTH YE</pre>  
|-
|-
!align=left |Length
!align=left {{table heading style}} |Length
||
||
242
242
|-
|-
!align=left |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
||
||
27.391 kDa
27.391 kDa
|-
|-
!align=left |pI
!align=left {{table heading style}} |pI
||
||
10.2 (calculated)
10.2 (calculated)
|-
|-
!align=left |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
||
||
24,410 - 24,660 (calc based on 9 Y, 2 W, and 2 C residues)
24,410 - 24,660 (calc based on 9 Y, 2 W, and 2 C residues)
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|-
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
80..226
|
[http://pfam.janelia.org/family/PF02565 PF02565  Recombination protein O C terminal]
|
 
|
PMID:19920124
|-
|
Domain
|
1..78
|
[http://pfam.janelia.org/family/PF11967 PF11967  Recombination protein O N terminal]
|
 
|
PMID:19920124


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|-
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!align=left |Structures
!align=left {{table heading style}} |Structures
||
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<beststructure>
<beststructure>
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</beststructure>
</beststructure>
|-
|-
!align=left |Models
!align=left {{table heading style}} |Models
||
||
View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0A7H3 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A7H3 PSI-PRED (P0A7H3)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A7H3 Genomes TO Protein (P0A7H3)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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!|Resource type!!Source!!Notes/Reference
!|Resource type!!Source!!Notes/Reference


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46aab025db195"  class=" tableEdit Gene_accessions_table"   
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16130490 GI:16130490]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_417060.1 GeneID:947038]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0008438
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10832-MONOMER EcoCyc:EG10832-MONOMER]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0A7H3 UniProtKB/Swiss-Prot:P0A7H3]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG10832
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG10832
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[[uniprot:P0A7H3|UniProt:P0A7H3]]
[http://www.ncbi.nlm.nih.gov/gene?term=947038 GeneID:947038]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
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|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://pfam.sanger.ac.uk/family?acc=PF02565 PFAM:PF02565]
RegulonDB:ECK120000823
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
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|
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[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417060 RefSeq:NP_417060]
EchoBASE:EB0825
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0006281_!_DNA repair]][[Category:GO:0005737_!_cytoplasm]][[Category:GO:0006310_!_DNA recombination]][[Category:Complex:RecFOR complex]]

Latest revision as of 20:53, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecO

Synonyms

gap repair protein[1], B2565[2][1], RecO[2][1] , ECK2563, JW2549, b2565

Product description

protein interacts with RecR and possibly RecF proteins[2][3];

Component of RecFOR complex[2][3]

Conjugational recombination and repair; DNA-binding protein; RecA-like strand assimilation[4]

EC number (for enzymes)


edit table


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006281

DNA repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00201

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003717

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00201

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003717

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of RecFOR complex

could be indirect

Protein

rpsE

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

slt

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

era

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

aegA

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

secD

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

rplO

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

rpsC

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

rpsG

PMID:16606699[5]

Experiment(s):EBI-1143287

Protein

rpsJ

PMID:19402753[6]

LCMS(ID Probability):99.6

edit table


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
edit table


Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MEGWQRAFVL HSRPWSETSL MLDVFTEESG RVRLVAKGAR SKRSTLKGAL QPFTPLLLRF
GGRGEVKTLR SAEAVSLALP LSGITLYSGL YINELLSRVL EYETRFSELF FDYLHCIQSL
AGVTGTPEPA LRRFELALLG HLGYGVNFTH CAGSGEPVDD TMTYRYREEK GFIASVVIDN
KTFTGRQLKA LNAREFPDAD TLRAAKRFTR MALKPYLGGK PLKSRELFRQ FMPKRTVKTH YE
Length

242

Mol. Wt

27.391 kDa

pI

10.2 (calculated)

Extinction coefficient

24,410 - 24,660 (calc based on 9 Y, 2 W, and 2 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

80..226

PF02565 Recombination protein O C terminal

PMID:19920124[7]

Domain

1..78

PF11967 Recombination protein O N terminal

PMID:19920124[7]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recO taxon=562,83333 </beststructure>

Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130490

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947038

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008438

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7H3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10832

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10832

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947038

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000823

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0825

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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