dnaE:Gene Product(s): Difference between revisions
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!align=left {{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
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DNA polymerase III alpha subunit<ref name='LIB:Riley_2006'/>, B0184<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, SdgC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, PolC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, DnaE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> | ''DNA polymerase III alpha subunit<ref name='LIB:Riley_2006'/>, B0184<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, SdgC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, PolC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, DnaE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK0183, JW0179, polC, sdgC, b0184'' | ||
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!align=left {{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
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****************************************************************************************** -->{{Product_GO_help}} | ****************************************************************************************** -->{{Product_GO_help}} | ||
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!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
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!|Partner Type!!Partner!!Notes!!References!!Evidence | !|Partner Type!!Partner!!Notes!!References!!Evidence | ||
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Protein | Protein | ||
| | | | ||
rpsA | |||
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Protein | Protein | ||
| | | | ||
dnaN | |||
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PMID:19402753 | PMID:19402753 | ||
| | | | ||
MALDI(Z-score):26.949307 | |||
|- | |- | ||
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Protein | Protein | ||
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rplU | |||
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Protein | Protein | ||
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tpiA | |||
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Protein | Protein | ||
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rpsP | |||
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Protein | Protein | ||
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rplM | |||
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Protein | Protein | ||
| | | | ||
tufB | |||
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PMID:19402753 | PMID:19402753 | ||
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MALDI(Z-score):19.758845 | |||
|- | |- | ||
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Protein | Protein | ||
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rplA | |||
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Protein | Protein | ||
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ssb | |||
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Protein | Protein | ||
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holA | |||
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Protein | Protein | ||
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holC | |||
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Protein | Protein | ||
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dnaQ | |||
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Protein | Protein | ||
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holE | |||
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Protein | Protein | ||
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holB | |||
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PMID:19402753 | PMID:19402753 | ||
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MALDI(Z-score):39.020174 | |||
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Protein | Protein | ||
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dnaX | |||
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Protein | Protein | ||
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holD | |||
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Protein | Protein | ||
| | | | ||
asnB | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
LCMS(ID Probability):99.0 | |||
|- | |- | ||
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Protein | Protein | ||
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rplL | |||
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PMID:19402753 | PMID:19402753 | ||
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LCMS(ID Probability):99. | LCMS(ID Probability):99.6 | ||
|- | |||
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Protein | |||
| | |||
Subunits of [[:Category:Complex:DNA polymerase III, holoenzyme|DNA polymerase III, holoenzyme]] | |||
| | |||
could be indirect | |||
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!|Compartment!!Description!!Evidence!!Source!!Notes | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
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!align=left {{table heading style}} |Name | |||
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!|Type!!Residues!!Description!!Notes!!References | !|Type!!Residues!!Description!!Notes!!References | ||
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View models at: | View models at: | ||
* [http:// | * [http://www.proteinmodelportal.org/query/uniprot/Q9JMR9 Protein Model Portal] | ||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P10443 PSI-PRED (P10443)] | |||
* [http://bioinf.cs.ucl.ac.uk/ | * [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P10443 Genomes TO Protein (P10443)] (GTOP) | ||
* [http://spock.genes.nig.ac.jp/~genome/ | |||
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!|Resource type!!Source!!Notes/Reference | !|Resource type!!Source!!Notes/Reference | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46aa22187c704" class=" tableEdit Gene_accessions_table" | ||
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!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/protein/16128177 GI:16128177] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/NP_414726.1 GeneID:944877] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
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[[ASAP]] | |||
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ASAP:ABE-0000624 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
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[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | |||
| | |||
[http://www.uniprot.org/uniprot/P10443 UniProtKB/Swiss-Prot:P10443] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
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[[EcoCyc]] | |||
| | |||
EcoCyc:EG10238 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | |||
[[EcoGene]] | |||
| | |||
EcoGene:EG10238 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
| | | | ||
[http://www. | [http://www.ncbi.nlm.nih.gov/gene?term=944877 GeneID:944877] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120000232 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0234 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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!|Name!!URL!!Comments | !|Name!!URL!!Comments | ||
Latest revision as of 20:22, 17 February 2015
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
DnaE |
|---|---|
| Synonyms |
DNA polymerase III alpha subunit[1], B0184[2][1], SdgC[2][1], PolC[2][1], DnaE[2][1] , ECK0183, JW0179, polC, sdgC, b0184 |
| Product description |
DNA polymerase III, α subunit[2][3]; Component of DNA polymerase III, core enzyme[3]; DNA polymerase III, holoenzyme[3] DNA polymerase III, alpha subunit; suppressor of dnaG-Ts[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
NOT |
GO:0008408 |
3'-5' exonuclease activity |
IDA: Inferred from Direct Assay |
F |
complete | |||
| GO:0003887 |
DNA-directed DNA polymerase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003887 |
DNA-directed DNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003887 |
DNA-directed DNA polymerase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003887 |
DNA-directed DNA polymerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006260 |
DNA replication |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008408 |
3'-5' exonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008408 |
3'-5' exonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016779 |
nucleotidyltransferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0003887 |
DNA-directed DNA polymerase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of DNA polymerase III, core enzyme |
could be indirect |
||
|
Protein |
ssb |
Experiment(s):EBI-883487 | ||
|
Protein |
aceE |
Experiment(s):EBI-883487 | ||
|
Protein |
dnaX |
Experiment(s):EBI-883487, EBI-890781 | ||
|
Protein |
holA |
Experiment(s):EBI-883487, EBI-890781 | ||
|
Protein |
holB |
Experiment(s):EBI-883487, EBI-880223 | ||
|
Protein |
holC |
Experiment(s):EBI-883487, EBI-880166, EBI-885330, EBI-890781 | ||
|
Protein |
holD |
Experiment(s):EBI-883487, EBI-880133, EBI-890781 | ||
|
Protein |
rpsB |
Experiment(s):EBI-883487, EBI-890781 | ||
|
Protein |
tufA |
Experiment(s):EBI-883487, EBI-890781 | ||
|
Protein |
pta |
Experiment(s):EBI-1135853 | ||
|
Protein |
helD |
Experiment(s):EBI-1135853 | ||
|
Protein |
asnB |
Experiment(s):EBI-890781 | ||
|
Protein |
dnaK |
Experiment(s):EBI-890781 | ||
|
Protein |
hrpB |
Experiment(s):EBI-890781 | ||
|
Protein |
lpdA |
Experiment(s):EBI-890781 | ||
|
Protein |
nikD |
Experiment(s):EBI-890781 | ||
|
Protein |
rhsD |
Experiment(s):EBI-890781 | ||
|
Protein |
rplA |
Experiment(s):EBI-890781 | ||
|
Protein |
rplC |
Experiment(s):EBI-890781 | ||
|
Protein |
rplD |
Experiment(s):EBI-890781 | ||
|
Protein |
rplL |
Experiment(s):EBI-890781 | ||
|
Protein |
rplM |
Experiment(s):EBI-890781 | ||
|
Protein |
rplU |
Experiment(s):EBI-890781 | ||
|
Protein |
rpsA |
Experiment(s):EBI-890781 | ||
|
Protein |
rpsP |
Experiment(s):EBI-890781 | ||
|
Protein |
tpiA |
Experiment(s):EBI-890781 | ||
|
Protein |
ybdL |
Experiment(s):EBI-890781 | ||
|
Protein |
bcsA |
Experiment(s):EBI-890781 | ||
|
Protein |
rpsA |
LCMS(ID Probability):99.6 | ||
|
Protein |
dnaN |
MALDI(Z-score):26.949307 | ||
|
Protein |
rplU |
LCMS(ID Probability):99.6 | ||
|
Protein |
tpiA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsP |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplM |
LCMS(ID Probability):99.6 | ||
|
Protein |
tufB |
MALDI(Z-score):19.758845 | ||
|
Protein |
rplA |
LCMS(ID Probability):99.6 | ||
|
Protein |
ssb |
LCMS(ID Probability):96.3 MALDI(Z-score):30.794950 | ||
|
Protein |
holA |
LCMS(ID Probability):99.6 MALDI(Z-score):38.471077 | ||
|
Protein |
holC |
LCMS(ID Probability):99.6 MALDI(Z-score):22.027887 | ||
|
Protein |
dnaQ |
LCMS(ID Probability):99.6 MALDI(Z-score):36.181251 | ||
|
Protein |
holE |
LCMS(ID Probability):99.6 MALDI(Z-score):18.880290 | ||
|
Protein |
holB |
MALDI(Z-score):39.020174 | ||
|
Protein |
dnaX |
LCMS(ID Probability):99.6 MALDI(Z-score):28.891532 | ||
|
Protein |
holD |
LCMS(ID Probability):99.6 MALDI(Z-score):26.029135 | ||
|
Protein |
asnB |
LCMS(ID Probability):99.0 | ||
|
Protein |
rplL |
LCMS(ID Probability):99.6 | ||
|
Protein |
Subunits of DNA polymerase III, holoenzyme |
could be indirect |
| |
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
Cytoplasm |
Purification |
| ||
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSEPRFVHLR VHSDYSMIDG LAKTAPLVKK AAALGMPALA ITDFTNLCGL VKFYGAGHGA GIKPIVGADF NVQCDLLGDE LTHLTVLAAN NTGYQNLTLL ISKAYQRGYG AAGPIIDRDW LIELNEGLIL LSGGRMGDVG RSLLRGNSAL VDECVAFYEE HFPDRYFLEL IRTGRPDEES YLHAAVELAE ARGLPVVATN DVRFIDSSDF DAHEIRVAIH DGFTLDDPKR PRNYSPQQYM RSEEEMCELF ADIPEALANT VEIAKRCNVT VRLGEYFLPQ FPTGDMSTED YLVKRAKEGL EERLAFLFPD EEERLKRRPE YDERLETELQ VINQMGFPGY FLIVMEFIQW SKDNGVPVGP GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDQVIEHVAD MYGRDAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLIPPDPGMT LAKAFEAEPQ LPEIYEADEE VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DEEGKHPVTQ FDKSDVEYAG LVKFDFLGLR TLTIINWALE MINKRRAKNG EPPLDIAAIP LDDKKSFDML QRSETTAVFQ LESRGMKDLI KRLQPDCFED MIALVALFRP GPLQSGMVDN FIDRKHGREE ISYPDVQWQH ESLKPVLEPT YGIILYQEQV MQIAQVLSGY TLGGADMLRR AMGKKKPEEM AKQRSVFAEG AEKNGINAEL AMKIFDLVEK FAGYGFNKSH SAAYALVSYQ TLWLKAHYPA EFMAAVMTAD MDNTEKVVGL VDECWRMGLK ILPPDINSGL YHFHVNDDGE IVYGIGAIKG VGEGPIEAII EARNKGGYFR ELFDLCARTD TKKLNRRVLE KLIMSGAFDR LGPHRAALMN SLGDALKAAD QHAKAEAIGQ ADMFGVLAEE PEQIEQSYAS CQPWPEQVVL DGERETLGLY LTGHPINQYL KEIERYVGGV RLKDMHPTER GKVITAAGLV VAARVMVTKR GNRIGICTLD DRSGRLEVML FTDALDKYQQ LLEKDRILIV SGQVSFDDFS GGLKMTAREV MDIDEAREKY ARGLAISLTD RQIDDQLLNR LRQSLEPHRS GTIPVHLYYQ RADARARLRF GATWRVSPSD RLLNDLRGLI GSEQVELEFD |
| Length |
1,160 |
| Mol. Wt |
129.908 kDa |
| pI |
5.1 (calculated) |
| Extinction coefficient |
102,110 - 103,610 (calc based on 39 Y, 8 W, and 12 C residues) |
| edit table |
Domains/Motifs/Modification Sites
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
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Structure
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Structure figures
Notes
Gene Product Resources
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| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 Maki, H & Kornberg, A (1985) The polymerase subunit of DNA polymerase III of Escherichia coli. II. Purification of the alpha subunit, devoid of nuclease activities. J. Biol. Chem. 260 12987-92 PubMed
- ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 7.0 7.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 8.16 8.17 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 9.0 9.1 9.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0008408 ! 3'-5' exonuclease activity
- GO:0003887 ! DNA-directed DNA polymerase activity
- GO:0003676 ! nucleic acid binding
- GO:0003677 ! DNA binding
- GO:0003824 ! catalytic activity
- GO:0005737 ! cytoplasm
- GO:0006260 ! DNA replication
- GO:0016740 ! transferase activity
- GO:0016779 ! nucleotidyltransferase activity
- Proteins