cpxA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|  id="Y46aa0212377ee"  class=" tableEdit Gene_nomenclature_table" 
 
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
|
||
''cpxA''  
''cpxA''
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
|
||
 
Conjugative plasmid expression
|-
|-
!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK3904, b3911, JW3882, ecfB, eup, ssd, rssE, ecf''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
''ECK3904, b3911, JW3882, ecfB, eup, ssd, rssE, ecf''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
|-
 
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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='148355'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G46aa0212badb2"  class=" tableEdit Gene_location_table" 
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
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MG1655
MG1655
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88.4 minutes&nbsp;
88.4 minutes&nbsp;
|
|
MG1655: 4102998..4101625<br />{{GBrowseFigureByCoord|4100625|4103998|ecoli|MG1655}}
MG1655: 4102998..4101625<br /><gbrowseImage>
name=NC_000913:4101625..4102998
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10163 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10163 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|4100625|4103998|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|4100625|4103998|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|4101625|4102998|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|4101625|4102998|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10163 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10163 EcoCyc Sequence Dump]  
|-
|-
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[[REL606]]
|
 
|
NC_012967: 4084457..4083084<br /><gbrowseImage>
name=NC_012967:4083084..4084457
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|4083084|4084457|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4083084|4084457|FASTA Dump}}
|-
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[[BW2952]]
|
 
|
NC_012759: 3991294..3992667<br /><gbrowseImage>
name=NC_012759:3991294..3992667
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|3991294|3992667|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|NC_012759|3991294|3992667|FASTA Dump}}
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W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 3531706..3533079<br />{{GBrowseFigureByCoord|3530706|3534079|ecoli_W3110|W3110}}  
W3110: 3531706..3533079<br /><gbrowseImage>
name=NC_007779:3531706..3533079
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|3530706|3534079|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|3531706|3533079|FASTA Dump}}  
|-
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[[DH10B]]
|
 
|
DH10B: 4202695..4201322<br /><gbrowseImage>
name=NC_010473:4201322..4202695
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|3530706|3534079|GBrowse(W3110)}}  
{{GBrowseLink|DH10B|NC_010473|4198576|4205441|GBrowse(DH10B)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:3531706..3533079&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|DH10B|NC_010473|4198576|4205441|FASTA Dump}}
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===Notes===
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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====Notes====
====Notes====
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="L46aa0213cafef"  class=" tableEdit Gene_allele_table dataTable" 
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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|
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109080 CGSC10799]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109080 CGSC10799]<ref name='LIB:cgsc'/>  
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temperature sensitive
temperature sensitive
|
|
[[PMID:6998969]]<ref name=PMID:6998969/>
PMID:6998969
|
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[[cgscmutation:10943|CGSC:10943]]
[[cgscmutation:10943|CGSC:10943]]
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|
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[[cgscmutation:100386|CGSC:100386]]
[[cgscmutation:100386|CGSC:100386]]
|
|
   
   
|-
|
''cpxA2''
|
|
|
Resistant to
|
Resistance to Amikacin
|
PMID:2185221
|
Strain: AE2038
|
Figure 1
|-
|
''ecfB1''
|
|
|
Resistant to
|
Resistance to 90 micrograms/ml Kanamycin 
|
PMID:353502
|
Strain: GE444
|
See table 2.
|-
|
''cpxA'' in strains KEC1 - KEC30
|


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|
Carbon Utilization
|
Carbon utilization of L-Serine
|
PMID:6802799
|
* Parent strain: CU1008
**Strain: KEC1 - KEC20
 
*Parent Strain: W4977
**Strain: KEC21-KEC30
|
See table one and Results section
|-
|
''ssd'' in mutant strain KEC3
|
 
|
 
|
Resistant to
|
Resistant to Kanamycin
|
PMID:6802799
|
Strain: KEC3
|
see table 2
|-
|
''ssd'' in mutant strain KEC3
|
 
|
 
|
Resistant to
|
Resistant to Neomycin
|
PMID:6802799
|
Strain: KEC3
|
Table 2
|-
|
''ecfB1'' ''kanA10''
|
 
|
 
|
Resistant to
|
Resistance to Aminoglycoside Kanamycin
|
PMID:353502
|
Strain: GE442
 
Parental: AB663
|
See details for subclass A for Kanomycin [http://biocyc.org/META/NEW-IMAGE?type=COMPOUND&object=CPD-4821 here]
 
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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===Notes===


==Genetic Resources==
==Genetic Resources==
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JW3882
JW3882
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCACTCCGCTTATACAGCGGCAA  
Primer 2:CCACTCCGCTTATACAGCGGCAA  
|-
|-
|
[[:Category:Kohara_Phage:8B10|8B10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=540 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:4B6|4B6]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=541 Genobase]
|
PMID:3038334
|-  
|
|
[[yihO:Gene|zih-35::Tn10]]
[[yihO:Gene|zih-35::Tn10]]
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|
|
est. P1 cotransduction: 21% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 21% <ref name='LIB:Nichols_linked_Tn'/>  
|-
|-  
|
|
[[pflD:Gene|pflD501::Tn10]]
[[pflD:Gene|pflD501::Tn10]]
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CAG18477 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37152 CGSC7470]<ref name='LIB:cgsc'/>
CAG18477 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37152 CGSC7470]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 17% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 17% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).


Synonyms:zij-501::Tn10
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CAG18477 also carries metF79(Am) (CGSC).
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===Notes===
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10163 EcoCyc:EG10163]
EcoCyc:EG10163
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|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0161 EchoBASE:EB0161]
|
|-
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b3911 EcoliGenExpDB:b3911]
|
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C4863 ECOL199310CYC:C4863]
EcoGene:EG10163
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|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10163 EcoGene:EG10163]
RegulonDB:ECK120000159
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOO157/new-image?object=CPXA ECOO157CYC:CPXA]
[http://www.ncbi.nlm.nih.gov/gene?term=948405 GeneID:948405]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/908 CGSC:908]
EchoBASE:EB0161
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0012772&formSubmitReturn=1 ASAP:ABE-0012772] 
ASAP:ABE-0012772
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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===Categories===
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[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:cpx genes]]
[[Category:cpx genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:50, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cpxA

Mnemonic

Conjugative plasmid expression

Synonyms

ECK3904, b3911, JW3882, ecfB, eup, ssd, rssE, ecf[1][2]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.4 minutes 

MG1655: 4102998..4101625
<gbrowseImage> name=NC_000913:4101625..4102998 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4084457..4083084
<gbrowseImage> name=NC_012967:4083084..4084457 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3991294..3992667
<gbrowseImage> name=NC_012759:3991294..3992667 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3531706..3533079
<gbrowseImage> name=NC_007779:3531706..3533079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4202695..4201322
<gbrowseImage> name=NC_010473:4201322..4202695 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcpxA (Keio:JW3882)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10799[4]

cpxA2(ts)

temperature sensitive

PMID:6998969[5]

CGSC:10943

ΔcpxA771::kan

PMID:16738554[3]

CGSC:100386

cpxA2

Resistant to

Resistance to Amikacin

PMID:2185221[6]

Strain: AE2038

Figure 1

ecfB1

Resistant to

Resistance to 90 micrograms/ml Kanamycin

PMID:353502[7]

Strain: GE444

See table 2.

cpxA in strains KEC1 - KEC30

Carbon Utilization

Carbon utilization of L-Serine

PMID:6802799[8]

  • Parent strain: CU1008
    • Strain: KEC1 - KEC20
  • Parent Strain: W4977
    • Strain: KEC21-KEC30

See table one and Results section

ssd in mutant strain KEC3

Resistant to

Resistant to Kanamycin

PMID:6802799[8]

Strain: KEC3

see table 2

ssd in mutant strain KEC3

Resistant to

Resistant to Neomycin

PMID:6802799[8]

Strain: KEC3

Table 2

ecfB1 kanA10

Resistant to

Resistance to Aminoglycoside Kanamycin

PMID:353502[7]

Strain: GE442

Parental: AB663

See details for subclass A for Kanomycin here


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3882

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCATAGGCAGCTTAACCGCGCG

Primer 2:CCACTCCGCTTATACAGCGGCAA

8B10

Kohara Phage

Genobase

PMID:3038334[10]

4B6

Kohara Phage

Genobase

PMID:3038334[10]

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[4]

est. P1 cotransduction: 21% [11]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[4]

est. P1 cotransduction: 17% [11]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10163

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10163

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000159

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948405

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0161

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012772

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ McEwen, J & Silverman, P (1980) Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB. J. Bacteriol. 144 60-7 PubMed
  6. ↑ Rainwater, S & Silverman, PM (1990) The Cpx proteins of Escherichia coli K-12: evidence that cpxA, ecfB, ssd, and eup mutations all identify the same gene. J. Bacteriol. 172 2456-61 PubMed
  7. ↑ 7.0 7.1 Thorbjarnardóttir, SH et al. (1978) Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli. Mol. Gen. Genet. 161 89-98 PubMed
  8. ↑ 8.0 8.1 8.2 Newman, EB et al. (1982) L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants. J. Bacteriol. 150 710-5 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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