acrE:Gene: Difference between revisions
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|{{Sub}}[[#Sequence Features|Sequence Features]] | |{{Sub}}[[#Sequence Features|Sequence Features]] | ||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK3252, b3265, JW3233, G7979''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/> | ''ECK3252, b3265, JW3233, G7979''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>, envC'' | ||
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* | * | ||
****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9f19f63c0c" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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73.54 minutes | 73.54 minutes | ||
| | | | ||
MG1655: 3411886..3413043<br /> | MG1655: 3411886..3413043<br /><gbrowseImage> | ||
name=NC_000913:3411886..3413043 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10266 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10266 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|3410886|3414043|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|3411886|3413043|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10266 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10266 EcoCyc Sequence Dump] | ||
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NC_012967: 3341755..3342912<br /> | NC_012967: 3341755..3342912<br /><gbrowseImage> | ||
name=NC_012967:3341755..3342912 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink|REL606|NC_012967|3341755|3342912|GBrowse (REL606)}} | {{GBrowseLink|REL606|NC_012967|3341755|3342912|GBrowse (REL606)}} | ||
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NC_012759: 3299034..3300191<br /> | NC_012759: 3299034..3300191<br /><gbrowseImage> | ||
name=NC_012759:3299034..3300191 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink|BW2952| | {{GBrowseLink|BW2952|NC_012759|3299034|3300191|GBrowse (BW2952)}} | ||
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{{GBrowseFastaLink|BW2952| | {{GBrowseFastaLink|BW2952|NC_012759|3299034|3300191|FASTA Dump}} | ||
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W3110: 3413719..3414876<br /> | W3110: 3413719..3414876<br /><gbrowseImage> | ||
name=NC_007779:3413719..3414876 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|W3110|NC_007779|3412719|3415876|GBrowse(W3110)}} | ||
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{{GBrowseFastaLink| | {{GBrowseFastaLink|W3110|NC_007779|3413719|3414876|FASTA Dump}} | ||
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DH10B: 3509631..3510788<br /> | DH10B: 3509631..3510788<br /><gbrowseImage> | ||
name=NC_010473:3509631..3510788 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|3507317|3513102|GBrowse(DH10B)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|3507317|3513102|FASTA Dump}} | ||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a9f1a0239a1" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|- | |||
| | |||
Protein Binding Site (SO:0000410) | |||
| | |||
MG1655 | |||
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-289bps from the translational start site | |||
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[[Methods: ROMA|ROMA]] | |||
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PMID:15520470 | |||
| | |||
CRP binding site | |||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="V46a9f1a06bef5" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010722 acrE::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010722 acrE::Tn5KAN-I-SceI] (FB21091) | ||
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Insertion at nt 611 in Minus orientation | |||
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PMID: | PMID:15262929 | ||
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[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21091] | ||
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contains [[pKD46]] | |||
|- | |- | ||
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010722 acrE::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010722 acrE::Tn5KAN-I-SceI] (FB21092) | ||
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Insertion at nt 611 in Minus orientation | |||
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PMID: | PMID:15262929 | ||
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[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21092] | ||
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does not contain [[pKD46]] | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.17079.B4e04b6e2187bf--> | |||
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* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
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****************************************************************************************** -->{{Genetic_interaction_help}} | |||
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B4e04b6e2187bf" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17079.B4e04b6e2187bf&page=17079&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.17079.B4e04b6e2187bf--></protect> | |||
<!-- Genetic Interacdtions Notes --> | |||
===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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****************************************************************************************** -->{{Gene_resources_help}} | ****************************************************************************************** -->{{Gene_resources_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46a9f1a12b862" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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Primer 2:CCtTTCGATGCAGTATCTGCGGG | Primer 2:CCtTTCGATGCAGTATCTGCGGG | ||
|- | |||
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[[:Category:Kohara_Phage:6G3|6G3]] | |||
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Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=529 Genobase] | |||
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PMID:3038334 | |||
|- | |||
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[[:Category:Kohara_Phage:6G9|6G9]] | |||
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Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=530 Genobase] | |||
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PMID:3038334 | |||
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****************************************************************************************** -->{{Gene_accessions_help}} | ****************************************************************************************** -->{{Gene_accessions_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O46a9f1a1c722f" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[[EcoCyc]] | |||
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EcoCyc:EG10266 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[EcoGene]] | |||
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EcoGene:EG10266 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000260 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=947706 GeneID:947706] | ||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EchoBASE]] | |||
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EchoBASE:EB0262 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[ASAP]] | |||
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ASAP:ABE-0010722 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J46a70cf4383c5" class=" tableEdit Links_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Name!!URL!!Comments | !|Name!!URL!!Comments | ||
Latest revision as of 20:46, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
acrE |
|---|---|
| Mnemonic |
Acridine |
| Synonyms | |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
73.54 minutes |
MG1655: 3411886..3413043 |
||
|
NC_012967: 3341755..3342912 |
||||
|
NC_012759: 3299034..3300191 |
||||
|
W3110 |
|
W3110: 3413719..3414876 |
||
|
DH10B: 3509631..3510788 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Protein Binding Site (SO:0000410) |
MG1655 |
-289bps from the translational start site |
CRP binding site | ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔacrE (Keio:JW3233) |
deletion |
deletion |
|||||
|
acrE::Tn5KAN-I-SceI (FB21091) |
Insertion at nt 611 in Minus orientation |
contains pKD46 | |||||
|
acrE::Tn5KAN-I-SceI (FB21092) |
Insertion at nt 611 in Minus orientation |
does not contain pKD46 | |||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3233 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACGAAACATGCCAGGTTTTT Primer 2:CCtTTCGATGCAGTATCTGCGGG | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 11% [9] | ||
|
Linked marker |
est. P1 cotransduction: 91% [9] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Zheng, D et al. (2004) Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. Nucleic Acids Res. 32 5874-93 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).