Cis Element:Phage lambda attP: Difference between revisions

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===Notes===
===Notes===
In the free phage, the ''att'' site is referred to as ''attP'' (<u>p</u>hage <u>att</u>achment site) while the corresponding site in a host genome is called ''attB'' (<u>b</u>acterial <u>att</u>achment site).  After integration occurs to create a prophage, these sites are then known as ''attL'' (<u>l</u>eft end of prophage) and ''attR'' (<u>r</u>ight end of prophage).  Reviewed in <u>Lambda II Monograph 13</u>. Edited by ROGER W. HENDRIX, JEFFREY W. ROBERTS,FRANKLIN W. STAHL and ROBERT A. WEISBERG. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724. 1983
In the free phage, the ''att'' site is referred to as ''attP'' (<u>p</u>hage <u>att</u>achment site) while the corresponding site in a host genome is called ''attB'' (<u>b</u>acterial <u>att</u>achment site).  After integration occurs to create a prophage, these sites are then known as ''attL'' (<u>l</u>eft end of prophage) and ''attR'' (<u>r</u>ight end of prophage).  Reviewed in <u>Lambda II.</u><ref name=LIB:Lambda_II/>


== Location and Sequence ==
== Location and Sequence ==
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NC_001416: 27718..27746<br />{{GBrowseFigureByCoord|27713|27751|bacteriophage_lambda|NC_001416}}  
NC_001416: 27571..27813<br />{{GBrowseFigureByCoord|27521|27863|bacteriophage_lambda|NC_001416}}  
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{{GBrowseLink|bacteriophage_lambda|NC_001416|27718|27746|Gbrowse (Bacteriophage lambda)}}
{{GBrowseLink|bacteriophage_lambda|NC_001416|27571|27813|Gbrowse (Bacteriophage lambda)}}
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{{GBrowseFastaLink|bacteriophage_lambda|NC_001416|27718|27746|FASTA Dump}}  
{{GBrowseFastaLink|bacteriophage_lambda|NC_001416|27571|27813|FASTA Dump}}  


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!|Partner Type!!Partner!!Notes!!References!!Evidence
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Protein
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Phage Lambda Int protein
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PMID:159130, PMID:2958633
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Protein
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''E. coli'' host protein IHF (Integration Host Factor)
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PMID:2958633
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Protein
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Phage Lambda Xis protein
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PMID:3156374
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!|Database!!Accession!!Notes
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[[EcoCyc]]
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EcoCyc:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EcoGene]]
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EcoGene:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[RegulonDB]]
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RegulonDB:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EchoBASE]]
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EchoBASE:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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<references/>
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===Categories===[[Category:Phage lambda:Cis_Element]]
===Categories===
[[Category:Phage lambda:Cis_Element]]

Latest revision as of 08:40, 27 May 2013

Cis element summary table

Standard Name

lambda attP

Synonyms
Function

phage attachment site

Location

approximately nt 27 571 - 27 813 (PMID:6246439[1])

attP core sequence located at nt 27 721 - 27 735 (PMID:6246439[1])

SO terms


Notes

In the free phage, the att site is referred to as attP (phage attachment site) while the corresponding site in a host genome is called attB (bacterial attachment site). After integration occurs to create a prophage, these sites are then known as attL (left end of prophage) and attR (right end of prophage). Reviewed in Lambda II.[2]

Location and Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

Phage Lambda (NC_001416)

NC_001416: 27571..27813
gbrowse_img.php?coord=27521..27863&genome=bacteriophage_lambda&name=NC_001416&trackset=0&width=300&.png


Notes

Interactions

[back to top]


See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Phage Lambda Int protein

PMID:159130[3], PMID:2958633[4]

Protein

E. coli host protein IHF (Integration Host Factor)

PMID:2958633[4]

Protein

Phage Lambda Xis protein

PMID:3156374[5]



Notes

Mutant alleles and phenotypes

[back to top]


Allele Nt change(s) Phenotype: Type Phenotype: Description Reference Availability Comments


Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Notes

Accessions

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655


Notes

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 Hsu, PL et al. (1980) The lambda phage att site: functional limits and interaction with Int protein. Nature 285 85-91 PubMed
  2. ↑ Edited by Roger W. Hendrix, Jeffrey W. Roberts, Franklin W. Stahl and Robert A. Weisberg (1983) Lambda II (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)
  3. ↑ Ross, W et al. (1979) Interaction of int protein with specific sites on lambda att DNA. Cell 18 297-307 PubMed
  4. ↑ 4.0 4.1 Thompson, JF et al. (1987) Protein-protein interactions in a higher-order structure direct lambda site-specific recombination. J. Mol. Biol. 195 481-93 PubMed
  5. ↑ Yin, S et al. (1985) Interaction of the lambda site-specific recombination protein Xis with attachment site DNA. Proc. Natl. Acad. Sci. U.S.A. 82 1040-4 PubMed

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