PMID:2958633
| Citation |
Thompson, JF, de Vargas, LM, Skinner, SE and Landy, A (1987) Protein-protein interactions in a higher-order structure direct lambda site-specific recombination. J. Mol. Biol. 195:481-93 |
|---|---|
| Abstract |
The highly directional site-specific recombination of bacteriophage lambda is tightly regulated by the binding of three different proteins to a complex array of sites. The manner in which these reactions are both stimulated and inhibited by co-operative binding of proteins to specific sites on the P arm of attP and AttR has been elucidated by correlation of nuclease protection with recombination studies of both wild-type and mutant DNAs. In addition to co-operative forces, there is a specific competitive interaction that allows the protein-DNA complex to serve as a "biological switch". This switch does not depend upon the simple occlusion of DNA binding sites by neighboring proteins; but, rather, the outcome of this competition is dependent on long-range interactions that vary between the higher-order structures of attP and attR. These higher-order structures are dependent on cooperative interactions involving three proteins binding to five or more sites. |
| Links | |
| Keywords |
Allosteric Site; Bacterial Proteins/metabolism; Bacteriophage lambda/genetics; Binding Sites; DNA, Recombinant/metabolism; DNA, Viral/metabolism; DNA-Binding Proteins/metabolism; Integration Host Factors; Mutation; Viral Core Proteins/metabolism; Viral Proteins/metabolism |
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