Cis Element:Phage lambda attP

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Cis element summary table

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Standard Name

lambda attP

Synonyms
Function

phage attachment site

Location

approximately nt 27 571 - 27 813 (PMID:6246439[1])

attP core sequence located at nt 27 721 - 27 735 (PMID:6246439[1])

SO terms


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Notes

In the free phage, the att site is referred to as attP (phage attachment site) while the corresponding site in a host genome is called attB (bacterial attachment site). After integration occurs to create a prophage, these sites are then known as attL (left end of prophage) and attR (right end of prophage). Reviewed in Lambda II.[2]

Location and Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

Phage Lambda (NC_001416)

NC_001416: 27571..27813
gbrowse_img.php?coord=27521..27863&genome=bacteriophage_lambda&name=NC_001416&trackset=0&width=300&.png

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Notes

Interactions

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Partner Type Partner Notes References Evidence

Protein

Phage Lambda Int protein

PMID:159130[3], PMID:2958633[4]

Protein

E. coli host protein IHF (Integration Host Factor)

PMID:2958633[4]

Protein

Phage Lambda Xis protein

PMID:3156374[5]


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Notes

Mutant alleles and phenotypes

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Allele Nt change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

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Notes

Accessions

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

References

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  1. 1.0 1.1 Hsu, PL et al. (1980) The lambda phage att site: functional limits and interaction with Int protein. Nature 285 85-91 PubMed
  2. Edited by Roger W. Hendrix, Jeffrey W. Roberts, Franklin W. Stahl and Robert A. Weisberg (1983) Lambda II (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)
  3. Ross, W et al. (1979) Interaction of int protein with specific sites on lambda att DNA. Cell 18 297-307 PubMed
  4. 4.0 4.1 Thompson, JF et al. (1987) Protein-protein interactions in a higher-order structure direct lambda site-specific recombination. J. Mol. Biol. 195 481-93 PubMed
  5. Yin, S et al. (1985) Interaction of the lambda site-specific recombination protein Xis with attachment site DNA. Proc. Natl. Acad. Sci. U.S.A. 82 1040-4 PubMed

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