yfaS:Gene

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yfaS

Mnemonic
Synonyms

Note that this is a pseudogene in K12 strains., ECK2220, JW2221, JW2222, yfaR, b4500

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

50.17 minutes 

MG1655: 2328305..2327820
<gbrowseImage> name=NC_000913:2327820..2328305 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2334953..2334468
<gbrowseImage> name=NC_007779:2334468..2334953 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

50.18 minutes 

MG1655: 2332424..2328321
<gbrowseImage> name=NC_000913:2328321..2332424 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2339072..2334969
<gbrowseImage> name=NC_007779:2334969..2339072 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

 

MG1655: 2332424..2327820
<gbrowseImage> name=NC_000913:2327820..2332424 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2339072..2334468
<gbrowseImage> name=NC_007779:2334468..2339072 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyfaS782::kan

PMID:16738554[1]

CGSC:104624

ΔyfaS781::kan

PMID:16738554[1]

CGSC:104622


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[2]

Status:

Primer 1:

Primer 2:

5A1

Kohara Phage

Genobase

PMID:3038334[3]

4F12

Kohara Phage

Genobase

PMID:3038334[3]

E13A5

Kohara Phage

Genobase

PMID:3038334[3]

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[4]

est. P1 cotransduction: 75% [5]
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[4]

est. P1 cotransduction: 60% [5]
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2228

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2770

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z3481

ASAP (EcoliWikiPage)

ASAP:ABE-0007364

NCBI (EcoliWiki Page)

GeneID:1450231

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003732

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  2. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. 3.0 3.1 3.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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