ubiH:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ubiH

Mnemonic

Ubiquinone

Synonyms

ECK2902, b2907, JW2875, visB, acd[1], acd

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

65.75 minutes 

MG1655: 3051540..3050362
<gbrowseImage> name=NC_000913:3050362..3051540 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2939269..2938091
<gbrowseImage> name=NC_012967:2938091..2939269 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2937510..2938688
<gbrowseImage> name=NC_012759:2937510..2938688 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3052174..3050996
<gbrowseImage> name=NC_007779:3050996..3052174 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3145410..3144232
<gbrowseImage> name=NC_010473:3144232..3145410 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ubiH(del) (Keio:JW2875)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10230[3]

ubiH101

CGSC:7477

ubiH758(del)::kan

PMID:16738554[2]

CGSC:103272

visB103

Sensitivity to

visible light (460nm)

PMID:1339425[4]

Strains VS550 and VS551

visB(del)

deletion

deletion

Sensitivity to

visible light (460nm)

PMID:1339425[4]

Strain VS560 and VS005

visB(del):KmR ubiA

deletion

deletion

not photosensitive

PMID:1339425[4]

Strain VS561

ubiH758(del)::FRT

deletion

Mutagenesis Rate

Decrease in Stress Induced Mutagenesis (SIM)

PMID:23224554[5]

Parent Strain: SMR4562 Experimental Strain: SMR12260

The mutation caused a strong decrease in the level of SIM, over 90 percent decrease of the Wild Type levels. See Table S3 for full experimental results.

ubiH758(del)::FRT

deletion

Sensitivity to

Increase in SDS-EDTA sensitivity

PMID:23224554[5]

Parent Strain: SMR4562 Experimental Strain: SMR12260

SDS-EDTA sensitivity increased relative toward the wild type. See table S7 and S1 for a summary of experimental results.

SMR4562 yiaG-yfp FRTcatFRT ubiH758(del)::FRTKanFRT

Deletion

Sigma S activity

Decrease in SigmaS activity

PMID:23224554[5]

Parental Strain: SMR10582 Experimental Strain: SMR12686

See table S8 for full experimental strain

CAG45114 ubiH758(del)::FRTKanFRT

deletion

SigmaE activity

Decrease in SigmaE activity

PMID:23224554[5]

Parental Strain: CAG45114 Experimental Strain: SMR15295

See table S11 for full experimental calculations.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2875

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGCGTAATCATCGTCGGTGG

Primer 2:CCACGCGCCACCCAACCGAGGGT

5E11

Kohara Phage

Genobase

PMID:3038334[7]

10B4

Kohara Phage

Genobase

PMID:3038334[7]

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[3]

est. P1 cotransduction: % [8]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[3]

est. P1 cotransduction: 24% [8]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11324

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11324

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001295

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947388

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1300

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009545

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 Nakahigashi, K et al. (1992) Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone. J. Bacteriol. 174 7352-9 PubMed
  5. 5.0 5.1 5.2 5.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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