ubiH:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ubiH |
|---|---|
| Mnemonic |
Ubiquinone |
| Synonyms |
ECK2902, b2907, JW2875, visB, acd[1], acd |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
65.75 minutes |
MG1655: 3051540..3050362 |
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NC_012967: 2939269..2938091 |
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NC_012759: 2937510..2938688 |
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W3110 |
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W3110: 3052174..3050996 |
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DH10B: 3145410..3144232 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ubiH(del) (Keio:JW2875) |
deletion |
deletion |
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ubiH101 |
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ubiH758(del)::kan |
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visB103 |
Sensitivity to |
visible light (460nm) |
Strains VS550 and VS551 | ||||
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visB(del) |
deletion |
deletion |
Sensitivity to |
visible light (460nm) |
Strain VS560 and VS005 | ||
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visB(del):KmR ubiA |
deletion |
deletion |
not photosensitive |
Strain VS561 | |||
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ubiH758(del)::FRT |
deletion |
Mutagenesis Rate |
Decrease in Stress Induced Mutagenesis (SIM) |
Parent Strain: SMR4562 Experimental Strain: SMR12260 |
The mutation caused a strong decrease in the level of SIM, over 90 percent decrease of the Wild Type levels. See Table S3 for full experimental results. | ||
|
ubiH758(del)::FRT |
deletion |
Sensitivity to |
Increase in SDS-EDTA sensitivity |
Parent Strain: SMR4562 Experimental Strain: SMR12260 |
SDS-EDTA sensitivity increased relative toward the wild type. See table S7 and S1 for a summary of experimental results. | ||
|
SMR4562 yiaG-yfp FRTcatFRT ubiH758(del)::FRTKanFRT |
Deletion |
Sigma S activity |
Decrease in SigmaS activity |
Parental Strain: SMR10582 Experimental Strain: SMR12686 |
See table S8 for full experimental strain | ||
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CAG45114 ubiH758(del)::FRTKanFRT |
deletion |
SigmaE activity |
Decrease in SigmaE activity |
Parental Strain: CAG45114 Experimental Strain: SMR15295 |
See table S11 for full experimental calculations. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2875 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGCGTAATCATCGTCGGTGG Primer 2:CCACGCGCCACCCAACCGAGGGT | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: % [8] | ||
|
Linked marker |
est. P1 cotransduction: 24% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 4.2 Nakahigashi, K et al. (1992) Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone. J. Bacteriol. 174 7352-9 PubMed
- ↑ 5.0 5.1 5.2 5.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).