ubiE:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ubiE

Mnemonic

Ubiquinone

Synonyms

ECK3827, b3833, JW5581, yigO[1], menG, menH, yigO

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

86.58 minutes 

MG1655: 4016878..4017633
<gbrowseImage> name=NC_000913:4016878..4017633 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3997326..3998081
<gbrowseImage> name=NC_012967:3997326..3998081 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3906547..3907302
<gbrowseImage> name=NC_012759:3906547..3907302 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3617826..3617071
<gbrowseImage> name=NC_007779:3617071..3617826 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4115798..4116553
<gbrowseImage> name=NC_010473:4115798..4116553 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ubiE(del) (Keio:JW5581)

deletion

deletion

PMID:16738554[2]

Shigen

ubiEG142D

G142D

In ubiE401; defective

seeded from UniProt:P0A887

ubiE778(del)::FRT

deletion

Mutagenesis rate

Decrease of Stress Induced Mutagenesis (SIM).

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12242

The Mutation conferred a strong decrease in SIM, with a decrease in mutant frequency by over 90 percent of the wild type levels. See table S3 for full experimental data.

ubiE778(del)::FRT

deletion

Sensitivity to

Increased Sensitivity toward SDS-EDTA

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12242

The mutation conferred an increase SDS-EDTA sensitivity. See tables S7 and S11 for experimental results.

SMR4562 ubiE778(del)::FRT yiaG-yfp FRTcatFRT

deletion

SigmaS activity

Decrease in SigmaS activity

PMID:23224554[3]

Parental Strain: SMR10582 Experimental Strain: SMR12657

See table S8 for full experimental results.

CAG45114 ubiE778(del)::FRTKanFRT

deletion

SigmaE activity

Decrease in sigmaE activity

PMID:23224554[3]

Parental Strain: CAG45114 Experimental Strain: SMR15257

See table S11 for full experimental data.

<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5581

Plasmid clone

Shigen

PMID:16769691[4]

Status:cloned as old JW

Primer 1:GCCGTGGATAAGTCACAAGAAAC

Primer 2:CCGAACTTATAACCACGATGCAG

16G1

Kohara Phage

Genobase

PMID:3038334[5]

1C10

Kohara Phage

Genobase

PMID:3038334[5]

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[6]

est. P1 cotransduction: 76% [7]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

fad-751::Tn10

Linked marker

CAG18496 = CGSC7465[6]

est. P1 cotransduction: 70% [7]
Synonyms:fadAB101::Tn10

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11473

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11473

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001435

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948926

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1441

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012534

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 3.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories