trmI:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

trmI

Mnemonic

tRNA methyltransferase

Synonyms

yggH, ECK2955, b2960, JW2927, trmB[1][2][3], trmB?

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

66.82 minutes 

MG1655: 3100874..3100155
<gbrowseImage> name=NC_000913:3100155..3100874 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2987794..2988513
<gbrowseImage> name=NC_012967:2987794..2988513 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2987303..2988022
<gbrowseImage> name=NC_012759:2987303..2988022 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3101508..3100789
<gbrowseImage> name=NC_007779:3100789..3101508 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3195943..3195224
<gbrowseImage> name=NC_010473:3195224..3195943 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

trmBN152A

N152A

No effect

seeded from UniProt:P0A8I5

trmBE220A

E220A

Reduces catalytic activity 10-fold

seeded from UniProt:P0A8I5

trmBR150A

R150A

Reduces catalytic activity about 3- fold

seeded from UniProt:P0A8I5

trmBR155A

R155A

Loss of activity

seeded from UniProt:P0A8I5

trmBD180A

D180A

Reduces catalytic activity over 10- fold

seeded from UniProt:P0A8I5

trmBH151A

H151A

Reduces catalytic activity over 10- fold

seeded from UniProt:P0A8I5

trmBT217A

T217A

Reduces catalytic activity over 10- fold

seeded from UniProt:P0A8I5

trmBR26A

R26A

Reduces catalytic activity over 10- fold

seeded from UniProt:P0A8I5

trmBD144A

D144A

Loss of activity

seeded from UniProt:P0A8I5

trmBR154A

R154A

Loss of activity

seeded from UniProt:P0A8I5

ΔyggH735::kan

PMID:16738554[4]

CGSC:104996

trmB1

CGSC:13081


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[5]

Status:

Primer 1:

Primer 2:

12C6

Kohara Phage

Genobase

PMID:3038334[6]

3A9

Kohara Phage

Genobase

PMID:3038334[6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11779

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11779

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001713

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947448

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1727

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009714

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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