topA:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
TopA |
|---|---|
| Synonyms |
DNA topoisomerase I, omega subunit[1], B1274[2][1], SupX[2][1], TopA[2][1], Topo I[2][1], DNA topoisomerase I (ω protein)[2][1] , asuA?, ECK1268, JW1266, supX, b1274 |
| Product description |
Topoisomerase I[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
IGI: Inferred from Genetic Interaction |
EcoliWiki:parC|EcoliWiki:gyrA |
P |
complete | |||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003917 |
DNA topoisomerase type I activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003917 |
DNA topoisomerase type I activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003917 |
DNA topoisomerase type I activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006259 |
DNA metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0006268 |
DNA unwinding involved in replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006268 |
DNA unwinding involved in replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016853 |
isomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046872 |
metal ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
ssb |
Experiment(s):EBI-888130 | ||
|
Protein |
aceF |
Experiment(s):EBI-888130, EBI-893705 | ||
|
Protein |
alaS |
Experiment(s):EBI-888130 | ||
|
Protein |
macA |
Experiment(s):EBI-888130 | ||
|
Protein |
hns |
Experiment(s):EBI-888130 | ||
|
Protein |
lpdA |
Experiment(s):EBI-888130 | ||
|
Protein |
malP |
Experiment(s):EBI-888130 | ||
|
Protein |
pssA |
Experiment(s):EBI-888130 | ||
|
Protein |
recJ |
Experiment(s):EBI-888130 | ||
|
Protein |
rob |
Experiment(s):EBI-888130 | ||
|
Protein |
rplB |
Experiment(s):EBI-888130 | ||
|
Protein |
rplC |
Experiment(s):EBI-888130, EBI-893705 | ||
|
Protein |
rplS |
Experiment(s):EBI-888130 | ||
|
Protein |
rplV |
Experiment(s):EBI-888130, EBI-893705 | ||
|
Protein |
rpoA |
Experiment(s):EBI-888130 | ||
|
Protein |
rpoB |
Experiment(s):EBI-888130 | ||
|
Protein |
rpoC |
Experiment(s):EBI-888130, EBI-893705 | ||
|
Protein |
rpsC |
Experiment(s):EBI-888130 | ||
|
Protein |
rpsE |
Experiment(s):EBI-888130 | ||
|
Protein |
rpsG |
Experiment(s):EBI-888130 | ||
|
Protein |
rpsM |
Experiment(s):EBI-888130 | ||
|
Protein |
rstA |
Experiment(s):EBI-888130 | ||
|
Protein |
srmB |
Experiment(s):EBI-888130 | ||
|
Protein |
rluB |
Experiment(s):EBI-888130 | ||
|
Protein |
yciA |
Experiment(s):EBI-1139424 | ||
|
Protein |
rplS |
Experiment(s):EBI-1139424 | ||
|
Protein |
hfq |
Experiment(s):EBI-1139424 | ||
|
Protein |
rpsM |
Experiment(s):EBI-1139424 | ||
|
Protein |
rimJ |
Experiment(s):EBI-1139424 | ||
|
Protein |
gabP |
Experiment(s):EBI-893705 | ||
|
Protein |
hupA |
Experiment(s):EBI-893705 | ||
|
Protein |
hupB |
Experiment(s):EBI-893705 | ||
|
Protein |
kdgK |
Experiment(s):EBI-893705 | ||
|
Protein |
rfaL |
Experiment(s):EBI-893705 | ||
|
Protein |
rplM |
Experiment(s):EBI-893705 | ||
|
Protein |
rpmB |
Experiment(s):EBI-893705 | ||
|
Protein |
rpmG |
Experiment(s):EBI-893705 | ||
|
Protein |
rpoZ |
Experiment(s):EBI-893705 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-893705 | ||
|
Protein |
rpsT |
Experiment(s):EBI-893705 | ||
|
Protein |
ybiT |
Experiment(s):EBI-893705 | ||
|
Protein |
srmB |
MALDI(Z-score):23.372700 | ||
|
Protein |
rpmG |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplM |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsJ |
LCMS(ID Probability):99.6 | ||
|
Protein |
malP |
MALDI(Z-score):25.368226 | ||
|
Protein |
rpoB |
LCMS(ID Probability):98.2 MALDI(Z-score):13.655113 | ||
|
Protein |
rpoC |
LCMS(ID Probability):99.4 MALDI(Z-score):8.512704 | ||
|
Protein |
rpoZ |
LCMS(ID Probability):99.6 | ||
|
Protein |
rluB |
MALDI(Z-score):34.761880 | ||
|
Protein |
recJ |
MALDI(Z-score):18.199199 | ||
|
Protein |
rplB |
MALDI(Z-score):36.003310 | ||
|
Protein |
ssb |
MALDI(Z-score):34.039804 | ||
|
Protein |
rpoA |
LCMS(ID Probability):97.9 MALDI(Z-score):35.722433 | ||
| edit table |
</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK KDERGALVNR MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA WHLREVIGGD DARYSRVVFN EITKNAIRQA FNKPGELNID RVNAQQARRF MDRVVGYMVS PLLWKKIARG LSAGRVQSVA VRLVVERERE IKAFVPEEFW EVDASTTTPS GEALALQVTH QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP GAPFITSTLQ QAASTRLGFG VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE SPNQYASKEN SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVNKGDALT LVELTPAQHF TKPPARFSEA SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY DFTAQMENSL DQVANHEAEW KAVLDHFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP TCGRKMGIRT ASTGVFLGCS GYALPPKERC KTTINLVPEN EVLNVLEGED AETNALRAKR RCPKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR IKGYDGPIVE CEKCGSEMHL KMGRFGKYMA CTNEECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF VLRDGAAGVF LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTMVR FSRKTKQQYV SSEKDGKATG WSAFYVDGKW VEGKK |
| Length |
865 |
| Mol. Wt |
97.35 kDa |
| pI |
8.7 (calculated) |
| Extinction coefficient |
98,210 - 99,960 (calc based on 29 Y, 10 W, and 14 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Zechiedrich, EL et al. (2000) Roles of topoisomerases in maintaining steady-state DNA supercoiling in Escherichia coli. J. Biol. Chem. 275 8103-13 PubMed
- ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 6.26 6.27 6.28 6.29 6.30 6.31 6.32 6.33 6.34 6.35 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 7.0 7.1 7.2 7.3 7.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 9.0 9.1 9.2 9.3 9.4 9.5 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000166 ! nucleotide binding
- GO:0003676 ! nucleic acid binding
- GO:0003677 ! DNA binding
- GO:0003916 ! DNA topoisomerase activity
- GO:0006265 ! DNA topological change
- GO:0003917 ! DNA topoisomerase type I activity
- GO:0003918 ! DNA topoisomerase type II (ATP-hydrolyzing) activity
- GO:0005524 ! ATP binding
- GO:0005694 ! chromosome
- GO:0006259 ! DNA metabolic process
- GO:0006268 ! DNA unwinding involved in DNA replication
- GO:0008270 ! zinc ion binding
- GO:0016853 ! isomerase activity
- GO:0046872 ! metal ion binding
- Proteins
