thrS:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
ThrS |
|---|---|
| Synonyms |
threonyl-tRNA synthetase[1], B1719[2][1], ThrS[2][1] , ECK1717, JW1709, b1719 |
| Product description |
threonyl-tRNA synthetase[2][3]; Component of threonyl-tRNA synthetase[2][3] Threonine--tRNA ligase, autogenously regulated; binds Zn(II)[4] |
| EC number (for enzymes) |
6.1.1.3[1] |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003723 |
RNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0008270 |
zinc ion binding |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
EcoliWiki:thrS |
F |
ThrRS is a homodimer of thrS gene products. |
complete | ||
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006435 |
threonyl-tRNA aminoacylation |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0003723 |
RNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0004812 |
aminoacyl-tRNA ligase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0004829 |
threonine-tRNA ligase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0043039 |
tRNA aminoacylation |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0006418 |
tRNA aminoacylation for protein translation |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0004829 |
threonine-tRNA ligase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
EcoliWiki:thrS |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006435 |
threonyl-tRNA aminoacylation |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006435 |
threonyl-tRNA aminoacylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006435 |
threonyl-tRNA aminoacylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of threonyl-tRNA synthetase |
could be indirect |
||
|
Protein |
rplA |
Experiment(s):EBI-879795, EBI-884548 | ||
|
Protein |
dnaK |
Experiment(s):EBI-884548 | ||
|
Protein |
eno |
Experiment(s):EBI-884548 | ||
|
Protein |
ibpA |
Experiment(s):EBI-884548 | ||
|
Protein |
rpe |
Experiment(s):EBI-884548 | ||
|
Protein |
rplC |
Experiment(s):EBI-884548 | ||
|
Protein |
rplI |
Experiment(s):EBI-884548 | ||
|
Protein |
rplM |
Experiment(s):EBI-884548 | ||
|
Protein |
rplS |
Experiment(s):EBI-884548 | ||
|
Protein |
rplV |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsA |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsB |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsE |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsF |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsG |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsM |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsN |
Experiment(s):EBI-884548 | ||
|
Protein |
rpsT |
Experiment(s):EBI-884548 | ||
|
Protein |
wcaB |
Experiment(s):EBI-884548 | ||
|
Protein |
yhbY |
Experiment(s):EBI-884548 | ||
|
Protein |
yicC |
Experiment(s):EBI-884548 | ||
|
Protein |
recR |
Experiment(s):EBI-1140596 | ||
|
Protein |
nadE |
Experiment(s):EBI-1140596 | ||
|
Protein |
rpsC |
Experiment(s):EBI-1140596 | ||
|
Protein |
yggM |
Experiment(s):EBI-1140596 | ||
|
Protein |
rpsM |
LCMS(ID Probability):99.0 MALDI(Z-score):3.070866 | ||
|
Protein |
rplS |
LCMS(ID Probability):99.0 MALDI(Z-score):3.739354 | ||
|
Protein |
rplA |
LCMS(ID Probability):99.0 MALDI(Z-score):10.739046 | ||
|
Protein |
eno |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpe |
LCMS(ID Probability):99.6 | ||
|
Protein |
ibpA |
LCMS(ID Probability):99.6 | ||
|
Protein |
yicC |
LCMS(ID Probability):99.6 | ||
|
Protein |
wcaB |
LCMS(ID Probability):99.0 | ||
|
Small Molecule |
Borrelidin |
See figure 4. | ||
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MPVITLPDGS QRHYDHAVSP MDVALDIGPG LAKACIAGRV NGELVDACDL IENDAQLSII TAKDEEGLEI IRHSCAHLLG HAIKQLWPHT KMAIGPVIDN GFYYDVDLDR TLTQEDVEAL EKRMHELAEK NYDVIKKKVS WHEARETFAN RGESYKVSIL DENIAHDDKP GLYFHEEYVD MCRGPHVPNM RFCHHFKLMK TAGAYWRGDS NNKMLQRIYG TAWADKKALN AYLQRLEEAA KRDHRKIGKQ LDLYHMQEEA PGMVFWHNDG WTIFRELEVF VRSKLKEYQY QEVKGPFMMD RVLWEKTGHW DNYKDAMFTT SSENREYCIK PMNCPGHVQI FNQGLKSYRD LPLRMAEFGS CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE EQIRDEVNGC IRLVYDMYST FGFEKIVVKL STRPEKRIGS DEMWDRAEAD LAVALEENNI PFEYQLGEGA FYGPKIEFTL YDCLDRAWQC GTVQLDFSLP SRLSASYVGE DNERKVPVMI HRAILGSMER FIGILTEEFA GFFPTWLAPV QVVIMNITDS QSEYVNELTQ KLSNAGIRVK ADLRNEKIGF KIREHTLRRV PYMLVCGDKE VESGKVAVRT RRGKDLGSMD VNEVIEKLQQ EIRSRSLKQL EE |
| Length |
642 |
| Mol. Wt |
74.014 kDa |
| pI |
6.1 (calculated) |
| Extinction coefficient |
96,260 - 97,885 (calc based on 24 Y, 11 W, and 13 C residues) |
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Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 Sankaranarayanan, R et al. (1999) The structure of threonyl-tRNA synthetase-tRNA(Thr) complex enlightens its repressor activity and reveals an essential zinc ion in the active site. Cell 97 371-81 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Hennecke, H et al. (1977) Threonyl-transfer ribonucleic acid synthetase from Escherichia coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing lambda bacteriophage. J. Bacteriol. 131 943-50 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 8.0 8.1 8.2 8.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 9.0 9.1 9.2 9.3 9.4 9.5 9.6 9.7 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 10.0 10.1 10.2 10.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0005524 ! ATP binding
- GO:0003723 ! RNA binding
- GO:0008270 ! zinc ion binding
- GO:0005515 ! protein binding
- GO:0000166 ! nucleotide binding
- GO:0006435 ! threonyl-tRNA aminoacylation
- GO:0005737 ! cytoplasm
- GO:0004812 ! aminoacyl-tRNA ligase activity
- GO:0004829 ! threonine-tRNA ligase activity
- GO:0043039 ! tRNA aminoacylation
- GO:0006418 ! tRNA aminoacylation for protein translation
- Proteins
