spoT:Gene Product(s)

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SpoT

Synonyms

bifunctional (p)ppGpp synthetase II and guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase[1], B3650[2][1], SpoT[2][1] , ECK3640, JW3625, b3650

Product description

SPOT[2][3]

Guanosine 5'-diphosphate, 3'-diphosphate pyrophosphatase; ppGpp synthase II activity[4]

EC number (for enzymes)
edit table

<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003607

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0008893

guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.7.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0015969

guanosine tetraphosphate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004811

P

Seeded from EcoCyc (v14.0)

complete

GO:0015969

guanosine tetraphosphate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007685

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0030145

manganese ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0464

F

Seeded from EcoCyc (v14.0)

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplV

PMID:15690043[5]

Experiment(s):EBI-880676, EBI-886668

Protein

rpoC

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

rpsA

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

rpsC

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

rpsD

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

rpsG

PMID:15690043[5]

Experiment(s):EBI-880676, EBI-886668

Protein

rpsK

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

trmH

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

srmB

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

tolR

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

tufA

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

rluB

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

deaD

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

hrpA

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

mukB

PMID:15690043[5]

Experiment(s):EBI-880676

Protein

rplB

PMID:15690043[5]

Experiment(s):EBI-880676, EBI-886668

Protein

rplC

PMID:15690043[5]

Experiment(s):EBI-880676, EBI-886668

Protein

rplD

PMID:15690043[5]

Experiment(s):EBI-880676, EBI-886668

Protein

hupA

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplI

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplL

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplM

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplO

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplQ

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplR

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplS

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplU

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rplX

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpmB

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsB

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsE

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsF

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsH

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsI

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsJ

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsL

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsN

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsP

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsR

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsT

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

yubM

PMID:15690043[5]

Experiment(s):EBI-886668

Protein

rpsI

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rho

PMID:19402753[6]

MALDI(Z-score):37.631749

Protein

rpsH

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

acpP

PMID:19402753[6]

LCMS(ID Probability):99.4 MALDI(Z-score):28.517444

Protein

hupA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsT

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsJ

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpmB

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsP

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplR

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):3.518360

Protein

rplO

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753[6]

MALDI(Z-score):18.633215

Protein

rplU

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753[6]

LCMS(ID Probability):99.5

Protein

rplQ

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplI

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):5.990057

Protein

rplM

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplB

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):33.813246

Protein

rpsC

PMID:19402753[6]

MALDI(Z-score):35.014889

Protein

rplA

PMID:19402753[6]

MALDI(Z-score):27.617083

Protein

rpsD

PMID:19402753[6]

MALDI(Z-score):36.055397

Protein

cobB

PMID:19402753[6]

MALDI(Z-score):22.005263

Protein

srmB

PMID:19402753[6]

MALDI(Z-score):28.181346

Protein

rlmL

PMID:19402753[6]

MALDI(Z-score):18.735939

Protein

trmH

PMID:19402753[6]

MALDI(Z-score):20.760958

Protein

rplD

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):7.596467

Protein

pssA

PMID:19402753[6]

MALDI(Z-score):31.812602

Protein

rhlB

PMID:19402753[6]

MALDI(Z-score):18.111170

Protein

hfq

PMID:19402753[6]

LCMS(ID Probability):95.5 MALDI(Z-score):24.477043

Protein

deaD

PMID:19402753[6]

MALDI(Z-score):25.785939

Protein

rluB

PMID:19402753[6]

MALDI(Z-score):35.989620

Protein

nadK

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753[6]

LCMS(ID Probability):99.6

edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
edit table

<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MYLFESLNQL IQTYLPEDQI KRLRQAYLVA RDAHEGQTRS SGEPYITHPV AVACILAEMK
LDYETLMAAL LHDVIEDTPA TYQDMEQLFG KSVAELVEGV SKLDKLKFRD KKEAQAENFR
KMIMAMVQDI RVILIKLADR THNMRTLGSL RPDKRRRIAR ETLEIYSPLA HRLGIHHIKT
ELEELGFEAL YPNRYRVIKE VVKAARGNRK EMIQKILSEI EGRLQEAGIP CRVSGREKHL
YSIYCKMVLK EQRFHSIMDI YAFRVIVNDS DTCYRVLGQM HSLYKPRPGR VKDYIAIPKA
NGYQSLHTSM IGPHGVPVEV QIRTEDMDQM AEMGVAAHWA YKEHGETSTT AQIRAQRWMQ
SLLELQQSAG SSFEFIESVK SDLFPDEIYV FTPEGRIVEL PAGATPVDFA YAVHTDIGHA
CVGARVDRQP YPLSQPLTSG QTVEIITAPG ARPNAAWLNF VVSSKARAKI RQLLKNLKRD
DSVSLGRRLL NHALGGSRKL NEIPQENIQR ELDRMKLATL DDLLAEIGLG NAMSVVVAKN
LQHGDASIPP ATQSHGHLPI KGADGVLITF AKCCRPIPGD PIIAHVSPGK GLVIHHESCR
NIRGYQKEPE KFMAVEWDKE TAQEFITEIK VEMFNHQGAL ANLTAAINTT TSNIQSLNTE
EKDGRVYSAF IRLTARDRVH LANIMRKIRV MPDVIKVTRN RN
Length

702

Mol. Wt

79.343 kDa

pI

9.0 (calculated)

Extinction coefficient

54,780 - 55,780 (calc based on 22 Y, 4 W, and 8 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

45..144

PF01966 HD domain

PMID:19920124[7]

Domain

388..447

PF02824 TGS domain

PMID:19920124[7]

Domain

235..345

PF04607 Region found in RelA / SpoT proteins

PMID:19920124[7]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=spoT taxon=562,83333 </beststructure>

Models

View models at:

edit table
</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131521

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948159

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011935

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AG24

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10966

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10966

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948159

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000955

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0959

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 5.18 5.19 5.20 5.21 5.22 5.23 5.24 5.25 5.26 5.27 5.28 5.29 5.30 5.31 5.32 5.33 5.34 5.35 5.36 5.37 5.38 5.39 5.40 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 6.26 6.27 6.28 6.29 6.30 6.31 6.32 6.33 6.34 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 7.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

[back to top]


Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=spoT:Gene_Product(s)&oldid=1495431"