skp:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

HlpA

Synonyms

periplasmic chaperone[1], B0178[2][1], Skp[2][1], OmpH[2][1], HlpA[2][1], HLP-I[2][1] , ECK0177, hlpA, JW0173, ompH, b0178

Product description

periplasmic chaperone[2][3]

Periplasmic molecular chaperone for outer membrane proteins; trimeric[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0042597

periplasmic space

PMID:1838129[5]

C

Missing: evidence

GO:0005515

protein binding

PMID:17928002[6]

IDA: Inferred from Direct Assay

F

Skp is homotrimeric.

complete

GO:0006457

protein folding

PMID:10455120[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0030288

outer membrane-bounded periplasmic space

PMID:10455120[7]

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

glyS

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

hisS

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplE

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplI

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplM

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplO

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplS

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplU

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplV

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplX

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rpsM

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

spoT

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rnr

PMID:15690043[8]

Experiment(s):EBI-881988

Protein

rplU

PMID:19402753[9]

MALDI(Z-score):17.053160

Protein

rplX

PMID:19402753[9]

MALDI(Z-score):17.053160

Protein

hisS

PMID:19402753[9]

MALDI(Z-score):17.053160

Protein

Skp

Skp forms homotrimers


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Notes

Because Skp is a periplasmic chaperone, interaction data should be interpreted with caution. Many of the interactions reported large-scale interactome experiments using whole cell extracts [8] are with cytosolic proteins. Jachow et al [10] identified possible Skp substrates by examining interactions from periplasmic extracts.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

periplasm

From EcoCyc[3]

Periplasm

PMID:2167239[11]

EchoLocation:skp


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKKWLLAAGL GLALATSAQA ADKIAIVNMG SLFQQVAQKT GVSNTLENEF KGRASELQRM
ETDLQAKMKK LQSMKAGSDR TKLEKDVMAQ RQTFAQKAQA FEQDRARRSN EERGKLVTRI
QTAVKSVANS QDIDLVVDAN AVAYNSSDVK DITADVLKQV K
Length

161

Mol. Wt

17.687 kDa

pI

10.1 (calculated)

Extinction coefficient

6,990 (calc based on 1 Y, 1 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-20

UniProt Manual:Signal Peptides

UniProt:P0AEU7

Domain

6..161

PF03938 Outer membrane protein (OmpH-like)

PMID:19920124[12]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=skp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128171

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944861

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AEU7

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944861

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Thome, BM & Müller, M (1991) Skp is a periplasmic Escherichia coli protein requiring SecA and SecY for export. Mol. Microbiol. 5 2815-21 PubMed
  6. ↑ Qu, J et al. (2007) The trimeric periplasmic chaperone Skp of Escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. J. Mol. Biol. 374 91-105 PubMed
  7. ↑ 7.0 7.1 Schäfer, U et al. (1999) Skp, a molecular chaperone of gram-negative bacteria, is required for the formation of soluble periplasmic intermediates of outer membrane proteins. J. Biol. Chem. 274 24567-74 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. ↑ 9.0 9.1 9.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ Jarchow, S et al. (2008) Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp. Proteomics 8 4987-94 PubMed
  11. ↑ Thome, BM et al. (1990) A protein with sequence identity to Skp (FirA) supports protein translocation into plasma membrane vesicles of Escherichia coli. FEBS Lett. 269 113-6 PubMed
  12. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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