PMID:2167239
| Citation |
Thome, BM, Hoffschulte, HK, Schiltz, E and Müller, M (1990) A protein with sequence identity to Skp (FirA) supports protein translocation into plasma membrane vesicles of Escherichia coli. FEBS Lett. 269:113-6 |
|---|---|
| Abstract |
We have purified to homogeneity a 15 kDa-protein from a ribosomal salt extract of Escherichia coli that compensates in vitro a defect of SecA but not of SecB. Removal of this protein from a cell-free transcription/translation system impairs translocation into plasma membrane vesicles of the precursors of LamB and to a lesser degree also of OmpA. These results suggest a role of the 15 kDa-protein in bacterial protein export. The NH2-terminal 35 amino acids were found to be identical to those of the skp (firA) gene product, to which several putative functions have previously been attributed. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Biological Transport; Escherichia coli/metabolism; Genes, Bacterial; Molecular Sequence Data; Molecular Weight; Solubility |
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