sfsB:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
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| Standard name |
SfsB |
|---|---|
| Synonyms |
DNA-binding transcriptional activator of maltose metabolism[1], B3188[2][1], SfsB[2][1], Nlp[2][1] , ECK3177, JW3155, nlp, sfs7, b3188 |
| Product description |
Nlp transcriptional regulator[2][3] Overexpression increases maltose and lactose utilization; stimulates MalQ, MalP and LacZ[4] |
| EC number (for enzymes) |
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Notes
The sfsB gene was originally named nlp for Ner-like protein based on its amino acid sequence similarity to the DNA-binding domain of the Ner repressor from phage Mu. SfsB belongs to the family of Lambda repressor-like, DNA-binding proteins (IPR010982) so is likely to be a transcription factor. However, it's physiological role is not clear.
When present in multicopy, sfsB allows strain MK2001 to ferment maltose[5]. MK2001 is an E. coli K-12 strain that doesn't make cAMP because the cya gene is disrupted by insertion of a KmR cassette and that also carries the crp*1 mutation. The crp*1 allele allows a strain lacking cAMP to have a Lac+ phenotype on MacConkey lactose medium, but is still Mal- on MacConkey maltose medium [6]. The sfsB gene in multicopy does not confer a Mal+ on a cya::KmR crp+ strain.
A total of 12 different E. coli DNA fragments with this phenotype were isolated [7]. One other of the plasmid inserts, sfs1, was sequenced. The sfs1 gene was later renamed sfsA. Whether sfsA and sfsB are normally involved in regulating maltose catabolism has not been determined.
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
rpsD |
Experiment(s):EBI-1145105 | ||
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Protein |
ybaA |
Experiment(s):EBI-1145105 | ||
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Protein |
yneG |
Experiment(s):EBI-1145105 | ||
|
Protein |
rplB |
Experiment(s):EBI-1145105 | ||
|
Protein |
yghW |
Experiment(s):EBI-1145105 | ||
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Notes
Localization
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| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MESNFIDWHP ADIIAGLRKK GTSMAAESRR NGLSSSTLAN ALSRPWPKGE MIIAKALGTD PWVIWPSRYH DPQTHEFIDR TQLMRSYTKP KK |
| Length |
92 |
| Mol. Wt |
10.495 kDa |
| pI |
10.3 (calculated) |
| Extinction coefficient |
24,980 (calc based on 2 Y, 4 W, and 0 C residues) |
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Domains/Motifs/Modification Sites
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Structure
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Notes
Gene Product Resources
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| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Choi, YL et al. (1989) Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108. J. Bacteriol. 171 5222-5 PubMed
- ↑ Aiba, H et al. (1985) Mutations that alter the allosteric nature of cAMP receptor protein of Escherichia coli. EMBO J. 4 3329-32 PubMed
- ↑ Kawamukai, M et al. (1991) Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli. J. Bacteriol. 173 2644-8 PubMed
- ↑ 8.0 8.1 8.2 8.3 8.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
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