selB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SelB

Synonyms

selenocysteinyl-tRNA-specific translation factor[1], B3590[2][1], FdhA[2][1], SelB[2][1] , ECK3579, fdhA, JW3563, b3590

Product description

selenocysteinyl-tRNA-specific translation factor[2][3]

Elongation factor promoting selenocysteine incorporation; specific for selenocysteyl tRNA[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0001514

selenocysteine incorporation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004535

P

Seeded from EcoCyc (v14.0)

complete

GO:0001514

selenocysteine incorporation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015190

P

Seeded from EcoCyc (v14.0)

complete

GO:0001514

selenocysteine incorporation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015191

P

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004535

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015190

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015191

F

Seeded from EcoCyc (v14.0)

complete

GO:0003746

translation elongation factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004535

F

Seeded from EcoCyc (v14.0)

complete

GO:0003746

translation elongation factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015190

F

Seeded from EcoCyc (v14.0)

complete

GO:0003746

translation elongation factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015191

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004161

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004535

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015190

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015191

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004535

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015190

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015191

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsI

PMID:16606699[5]

Experiment(s):EBI-1146180

Protein

rplB

PMID:16606699[5]

Experiment(s):EBI-1146180

Protein

ompL

PMID:16606699[5]

Experiment(s):EBI-1146180

Protein

rplQ

PMID:16606699[5]

Experiment(s):EBI-1146180

Protein

aceE

PMID:15690043[6]

Experiment(s):EBI-883733

Protein

aceF

PMID:15690043[6]

Experiment(s):EBI-883733

Protein

dnaJ

PMID:15690043[6]

Experiment(s):EBI-883733, EBI-890918

Protein

pta

PMID:15690043[6]

Experiment(s):EBI-883733

Protein

rplB

PMID:15690043[6]

Experiment(s):EBI-883733

Protein

rplC

PMID:15690043[6]

Experiment(s):EBI-883733, EBI-890918

Protein

rpsD

PMID:15690043[6]

Experiment(s):EBI-883733, EBI-890918

Protein

srmB

PMID:15690043[6]

Experiment(s):EBI-883733, EBI-890918

Protein

rnr

PMID:15690043[6]

Experiment(s):EBI-883733

Protein

rluB

PMID:15690043[6]

Experiment(s):EBI-883733

Protein

yfiF

PMID:15690043[6]

Experiment(s):EBI-883733, EBI-890918

Protein

rho

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplA

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplD

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplI

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplL

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplO

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplS

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplT

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplU

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplV

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplX

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplY

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpmB

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpsA

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpsC

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpsE

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpsF

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpsG

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rpsT

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplM

PMID:15690043[6]

Experiment(s):EBI-890918

Protein

rplY

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplO

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):2.220191

Protein

rho

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplU

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

pta

PMID:19402753[7]

MALDI(Z-score):25.744061

Protein

rplT

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rpsF

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplI

PMID:19402753[7]

LCMS(ID Probability):99.4

Protein

rpsD

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):34.734394

Protein

srmB

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):15.619374

Protein

rplB

PMID:19402753[7]

LCMS(ID Probability):94.6 MALDI(Z-score):31.166264

Protein

rpsC

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

dnaJ

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):23.491939

Protein

rplA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):2.210716

Protein

yfiF

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):32.454436

Protein

rnr

PMID:19402753[7]

MALDI(Z-score):27.609239

Protein

rluB

PMID:19402753[7]

MALDI(Z-score):26.510533

Protein

rplL

PMID:19402753[7]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIIATAGHVD HGKTTLLQAI TGVNADRLPE EKKRGMTIDL GYAYWPQPDG RVPGFIDVPG
HEKFLSNMLA GVGGIDHALL VVACDDGVMA QTREHLAILQ LTGNPMLTVA LTKADRVDEA
RVDEVERQVK EVLREYGFAE AKLFITAATE GRGMDALREH LLQLPEREHA SQHSFRLAID
RAFTVKGAGL VVTGTALSGE VKVGDSLWLT GVNKPMRVRA LHAQNQPTET ANAGQRIALN
IAGDAEKEQI NRGDWLLADV PPEPFTRVIV ELQTHTPLTQ WQPLHIHHAA SHVTGRVSLL
EDNLAELVFD TPLWLADNDR LVLRDISARN TLAGARVVML NPPRRGKRKP EYLQWLASLA
RAQSDADALS VHLERGAVNL ADFAWARQLN GEGMRELLQQ PGYIQAGYSL LNAPVAARWQ
RKILDTLATY HEQHRDEPGP GRERLRRMAL PMEDEALVLL LIEKMRESGD IHSHHGWLHL
PDHKAGFSEE QQAIWQKAEP LFGDEPWWVR DLAKETGTDE QAMRLTLRQA AQQGIITAIV
KDRYYRNDRI VEFANMIRDL DQECGSTCAA DFRDRLGVGR KLAIQILEYF DRIGFTRRRG
NDHLLRDALL FPEK
Length

614

Mol. Wt

68.868 kDa

pI

6.6 (calculated)

Extinction coefficient

80,900 - 81,275 (calc based on 10 Y, 12 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..166

PF00009 Elongation factor Tu GTP binding domain

PMID:19920124[8]

Domain

189..257

PF03144 Elongation factor Tu domain 2

PMID:19920124[8]

Domain

423..480

PF09106 Elongation factor SelB, winged helix

PMID:19920124[8]

Domain

557..606

PF09107 Elongation factor SelB, winged helix

PMID:19920124[8]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=selB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131461

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948103

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011729

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P14081

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10942

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10942

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948103

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000931

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0935

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 6.26 6.27 6.28 6.29 6.30 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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