ribF:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RibF

Synonyms

bifunctional riboflavin kinase and FAD synthetase[1], B0025[2][1], YaaC[2][1], RibF[2][1] , ECK0026, JW0023, yaaC, b0025

Product description

RIBF[2]; bifunctional riboflavin kinase / FMN adenylyltransferase[3]

Riboflavin kinase and FAD synthase, riboflavin biosynthesis[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003919

FMN adenylyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002606

F

Seeded from EcoCyc (v14.0)

complete

GO:0003919

FMN adenylyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015864

F

Seeded from EcoCyc (v14.0)

complete

GO:0003919

FMN adenylyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0008531

riboflavin kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002606

F

Seeded from EcoCyc (v14.0)

complete

GO:0008531

riboflavin kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015865

F

Seeded from EcoCyc (v14.0)

complete

GO:0008531

riboflavin kinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.26

F

Seeded from EcoCyc (v14.0)

complete

GO:0009231

riboflavin biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002606

P

Seeded from EcoCyc (v14.0)

complete

GO:0009231

riboflavin biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015864

P

Seeded from EcoCyc (v14.0)

complete

GO:0009231

riboflavin biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015865

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

phnN

PMID:16606699[5]

Experiment(s):EBI-1135327

Protein

adhE

PMID:16606699[5]

Experiment(s):EBI-1135327

Protein

nadE

PMID:16606699[5]

Experiment(s):EBI-1135327

Protein

rplD

PMID:16606699[5]

Experiment(s):EBI-1135327

Protein

glpD

PMID:16606699[5]

Experiment(s):EBI-1135327

Protein

groL

PMID:16606699[5]

Experiment(s):EBI-1135327

Protein

aceE

PMID:15690043[6]

Experiment(s):EBI-889192

Protein

acrD

PMID:15690043[6]

Experiment(s):EBI-894280

Protein

recD

PMID:15690043[6]

Experiment(s):EBI-894280

Protein

recD

PMID:19402753[7]

LCMS(ID Probability):99.0

Protein

acrD

PMID:19402753[7]

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV MVMLFEPQPL
ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA ALTAQNFISD LLVKHLRVKF
LAVGDDFRFG AGREGDFLLL QKAGMEYGFD ITSTQTFCEG GVRISSTAVR QALADDNLAL
AESLLGHPFA ISGRVVHGDE LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV
ANIGTRPTVA GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE
LTAREFFGLT KPA
Length

313

Mol. Wt

34.734 kDa

pI

9.6 (calculated)

Extinction coefficient

7,450 - 7,950 (calc based on 5 Y, 0 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

184..308

PF01687 Riboflavin kinase

PMID:19920124[8]

Domain

11..168

PF06574 FAD synthetase

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ribF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128019

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000091

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AG40

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11079

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11079

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949129

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001067

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949129

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1071

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 5.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. 6.0 6.1 6.2 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  7. 7.0 7.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. 8.0 8.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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