purB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PurB

Synonyms

adenylosuccinate lyase[1], B1131[2][1], PurB[2][1] , ade, ECK1117, JW1117, b1131

Product description

ASL[2][3]

Adenylosuccinate lyase, purine synthesis[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004018

N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004769

F

Seeded from EcoCyc (v14.0)

complete

GO:0004018

N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013539

F

Seeded from EcoCyc (v14.0)

complete

GO:0004018

N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.3.2.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0004018

N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity

PMID:8969519[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0006164

purine nucleotide biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0658

P

Seeded from EcoCyc (v14.0)

complete

GO:0006188

IMP biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013539

P

Seeded from EcoCyc (v14.0)

complete

GO:0009152

purine ribonucleotide biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004769

P

Seeded from EcoCyc (v14.0)

complete

GO:0070626

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.3.2.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071[6]

IEP: Inferred from Expression Pattern

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

relA

PMID:16606699[7]

Experiment(s):EBI-1138972

Protein

narZ

PMID:16606699[7]

Experiment(s):EBI-1138972

Protein

clpX

PMID:16606699[7]

Experiment(s):EBI-1138972

Protein

nadE

PMID:16606699[7]

Experiment(s):EBI-1138972

Protein

aceE

PMID:15690043[8]

Experiment(s):EBI-880008

Protein

rplV

PMID:15690043[8]

Experiment(s):EBI-885051

Protein

rpsB

PMID:15690043[8]

Experiment(s):EBI-885051

Protein

ydaY

PMID:15690043[8]

Experiment(s):EBI-885051

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MELSSLTAVS PVDGRYGDKV SALRGIFSEY GLLKFRVQVE VRWLQKLAAH AAIKEVPAFA
ADAIGYLDAI VASFSEEDAA RIKTIERTTN HDVKAVEYFL KEKVAEIPEL HAVSEFIHFA
CTSEDINNLS HALMLKTARD EVILPYWRQL IDGIKDLAVQ YRDIPLLSRT HGQPATPSTI
GKEMANVAYR MERQYRQLNQ VEILGKINGA VGNYNAHIAA YPEVDWHQFS EEFVTSLGIQ
WNPYTTQIEP HDYIAELFDC VARFNTILID FDRDVWGYIA LNHFKQKTIA GEIGSSTMPH
KVNPIDFENS EGNLGLSNAV LQHLASKLPV SRWQRDLTDS TVLRNLGVGI GYALIAYQST
LKGVSKLEVN RDHLLDELDH NWEVLAEPIQ TVMRRYGIEK PYEKLKELTR GKRVDAEGMK
QFIDGLALPE EEKARLKAMT PANYIGRAIT MVDELK
Length

456

Mol. Wt

51.543 kDa

pI

5.9 (calculated)

Extinction coefficient

65,320 - 65,570 (calc based on 18 Y, 7 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

14..320

PF00206 Lyase

PMID:19920124[9]

Domain

331..445

PF08328 Adenylosuccinate lyase C-terminal

PMID:19920124[9]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129094

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945695

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003810

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AB89

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11314

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11314

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945695

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001285

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1290

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Green, SM et al. (1996) The purB gene of Escherichia coli K-12 is located in an operon. Microbiology (Reading, Engl.) 142 ( Pt 11) 3219-30 PubMed
  6. Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed
  7. 7.0 7.1 7.2 7.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. 8.0 8.1 8.2 8.3 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. 9.0 9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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