PMID:8969519
| Citation |
Green, SM, Malik, T, Giles, IG and Drabble, WT (1996) The purB gene of Escherichia coli K-12 is located in an operon. Microbiology (Reading, Engl.) 142 ( Pt 11):3219-30 |
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| Abstract |
The structural gene (purB) for succinyl-AMP (S-AMP) lyase and three additional ORFs are on the same DNA strand of the chromosome of Escherichia coli. Cassette mutagenesis and primer extension mapping demonstrated that purB is co-transcribed with an upstream gene (ORF23, or ycfC) encoding a 22.9 kDa membrane-associated protein of non-essential, but unknown, function unrelated to purine biosynthesis. The purB operon lies between phoP and an ORF expressing an essential function which may correspond to asuE (trmU). S-AMP lyase was purified to near homogeneity. The purified enzyme is a homotetramer of 50 kDa subunits, has a K(m) for S-AMP of 3.7 microM and a pH optimum of 7.4-7.6. |
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| Keywords |
Adenylosuccinate Lyase/chemistry; Adenylosuccinate Lyase/genetics; Adenylosuccinate Lyase/metabolism; Amino Acid Sequence; Base Sequence; Chromosomes, Bacterial/genetics; Cloning, Molecular; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Molecular Weight; Mutation; Open Reading Frames; Operon; Protein Conformation; Restriction Mapping; Signal Transduction; Transcription, Genetic |
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