priB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PriB

Synonyms

primosomal protein N[1], B4201[2][1] , ECK4197, JW4159, b4201

Product description

PriB[2][3];

Component of primosomal replication protein N[3]; primosome[3]

Primosomal protein n; ssDNA-binding protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003697

single-stranded DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00720

F

Seeded from EcoCyc (v14.0)

complete

GO:0003697

single-stranded DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000424

F

Seeded from EcoCyc (v14.0)

complete

GO:0005658

alpha DNA polymerase:primase complex

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0639

C

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00720

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0235

P

Seeded from EcoCyc (v14.0)

complete

GO:0006269

DNA replication, synthesis of RNA primer

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0639

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of primosomal replication protein N

could be indirect

Protein

ydcP

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

hns

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

rplB

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

dnaK

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

rplA

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

ppk

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

rpsG

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

rpsC

PMID:16606699[5]

Experiment(s):EBI-1147664

Protein

Subunits of primosome

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTNRLVLSGT VCRAPLRKVS PSGIPHCQFV LEHRSVQEEA GFHRQAWCQM PVIVSGHENQ
AITHSITVGS RITVQGFISC HKAKNGLSKM VLHAEQIELI DSGD
Length

104

Mol. Wt

11.441 kDa

pI

8.5 (calculated)

Extinction coefficient

5,500 - 6,000 (calc based on 0 Y, 1 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P07013

Domain

2..100

PF00436 Single-strand binding protein family

PMID:19920124[6]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=priB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16132023

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948722

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013742

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P07013

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10764

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10764

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948722

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000755

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0757

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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