prfB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PrfB

Synonyms

peptide chain release factor RF-2[1], B2891[2][1], SupK[2][1], PrfB[2][1] , ECK2886, JW5847, supK, b2891

Product description

peptide chain release factor RF2[2][3]

Peptide chain release factor 2, RF-2; translation termination factor recognizes UGA and UAA.[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003747

translation release factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000352

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00094

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004374

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005139

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020853

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006415

translational termination

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00094

P

Seeded from EcoCyc (v14.0)

complete

GO:0006415

translational termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000352

P

Seeded from EcoCyc (v14.0)

complete

GO:0006415

translational termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004374

P

Seeded from EcoCyc (v14.0)

complete

GO:0006415

translational termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005139

P

Seeded from EcoCyc (v14.0)

complete

GO:0006415

translational termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020853

P

Seeded from EcoCyc (v14.0)

complete

GO:0016149

translation release factor activity, codon specific

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00094

F

Seeded from EcoCyc (v14.0)

complete

GO:0016149

translation release factor activity, codon specific

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004374

F

Seeded from EcoCyc (v14.0)

complete

GO:0016149

translation release factor activity, codon specific

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005139

F

Seeded from EcoCyc (v14.0)

complete

GO:0016149

translation release factor activity, codon specific

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020853

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

ynfE

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

yeiT

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

ccmB

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

hupA

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

hupB

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

parE

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

polA

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

wecA

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

rplL

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

rplV

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

rpoA

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

rpsB

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

rpsE

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

ulaA

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

nagK

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

gcp

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

yjcF

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

rpsJ

PMID:15690043[5]

Experiment(s):EBI-889160

Protein

parE

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

yeiT

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

ynfE

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

nagK

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

ygjD

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

yjcF

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

rfe

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

ulaA

PMID:19402753[6]

LCMS(ID Probability):99.0

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MFEINPVNNR IQDLTERSDV LRGYLDYDAK KERLEEVNAE LEQPDVWNEP ERAQALGKER
SSLEAVVDTL DQMKQGLEDV SGLLELAVEA DDEETFNEAV AELDALEEKL AQLEFRRMFS
GEYDSADCYL DIQAGSGGTE AQDWASMLER MYLRWAESRG FKTEIIEESE GEVAGIKSVT
IKISGDYAYG WLRTETGVHR LVRKSPFDSG GRRHTSFSSA FVYPEVDDDI DIEINPADLR
IDVYRTSGAG GQHVNRTESA VRITHIPTGI VTQCQNDRSQ HKNKDQAMKQ MKAKLYELEM
QKKNAEKQAM EDNKSDIGWG SQIRSYVLDD SRIKDLRTGV ETRNTQAVLD GSLDQFIEAS
LKAGL
Length

365

Mol. Wt

41.25 kDa

pI

4.5 (calculated)

Extinction coefficient

43,890 - 44,140 (calc based on 11 Y, 5 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

85..195

PF03462 PCRF domain

PMID:19920124[7]

Domain

225..337

PF00472 RF-1 domain

PMID:19920124[7]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=prfB taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130793

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947369

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009488

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P07012

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10762

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10762

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947369

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000753

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0755

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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