phoQ:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
phoQ |
|---|---|
| Mnemonic |
Phosphate |
| Synonyms |
ECK1115, b1129, JW1115[1], JW1115 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
25.6 minutes |
MG1655: 1188999..1187539 |
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NC_012967: 1205146..1203686 |
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NC_012759: 1091464..1092924 |
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W3110 |
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W3110: 1191353..1189893 |
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DH10B: 1244444..1242984 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔphoQ (Keio:JW1115) |
deletion |
deletion |
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phoQD149A |
D149A |
Wild-type effect concerning mgrB transcription |
seeded from UniProt:P23837 | ||||
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phoQD90A |
D90A |
No significant effect (with or without MgCl(2) or CaCl(2)) |
seeded from UniProt:P23837 | ||||
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phoQEDDDDAE148QNNNNAQ |
EDDDDAE148QNNNNAQ |
Unable to bind divalent cations in vitro and impaired in the ability to respond to Mg(2+) deprivation in vivo |
seeded from UniProt:P23837 | ||||
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phoQD179L,A |
D179L,A |
Locked-on mutant defective in Mg(2+)-sensing and unable to control its phosphorylation state and phosphotransfer to phoP |
seeded from UniProt:P23837 | ||||
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phoQR439A |
R439A |
3-fold decrease in ATP affinity and 2-fold increase in activity |
seeded from UniProt:P23837 | ||||
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phoQK392A |
K392A |
44-fold decrease in ATP affinity and 6-fold decrease in activity |
seeded from UniProt:P23837 | ||||
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phoQR434A |
R434A |
2-fold decrease in ATP affinity and 51-fold decrease in activity |
seeded from UniProt:P23837 | ||||
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phoQD150I |
D150I |
Wild-type effect concerning mgrB transcription |
seeded from UniProt:P23837 | ||||
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phoQT47L |
T47L |
No significant effect (with or without MgCl(2) or CaCl(2)) |
seeded from UniProt:P23837 | ||||
|
phoQT48A,C,E,M,N,Q,S,V |
T48A,C,E,M,N,Q,S,V |
No significant effect (with or without MgCl(2) or CaCl(2)) |
seeded from UniProt:P23837 | ||||
|
phoQT48D,G,H,I,K,L,P,R |
T48D,G,H,I,K,L,P,R |
Confers less than 30% of the wild-type levels of phoQ/phoP- signaling cascade in absence of CaCl(2) or MgCl(2) |
seeded from UniProt:P23837 | ||||
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phoQT48F,W |
T48F,W |
Decreased sensitivity to repression by calcium but not by magnesium |
seeded from UniProt:P23837 | ||||
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phoQT48Y |
T48Y |
Higher activity than wild-type (with or without MgCl(2) or CaCl(2)) |
seeded from UniProt:P23837 | ||||
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phoQN68L |
N68L |
No significant effect (with or without MgCl(2) or CaCl(2)) |
seeded from UniProt:P23837 | ||||
|
phoQD151I |
D151I |
Wild-type effect concerning mgrB transcription |
seeded from UniProt:P23837 | ||||
|
phoQD152F |
D152F |
Wild-type effect concerning mgrB transcription |
seeded from UniProt:P23837 | ||||
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ΔphoQ789::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1115 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAAAATTACTGCGTCTTTT Primer 2:CCTTCATCTTTCGGCGCAGAATG | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 28% [6] | ||
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Linked marker |
est. P1 cotransduction: 42% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
