phnE:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

phnE

Mnemonic
Synonyms

ECK4096, b4103, JW4064, ECK4097, b4104, JW4065[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

93.12 minutes, 93.12 minutes, 93.12 minutes 

MG1655: 4320834..4320466
<gbrowseImage> name=NC_000913:4320466..4320834 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4302083..4301265
<gbrowseImage> name=NC_012967:4301265..4302083 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4327489..4327121
<gbrowseImage> name=NC_007779:4327121..4327489 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

93.12 minutes, 93.12 minutes, 93.12 minutes 

MG1655: 4321304..4320684
<gbrowseImage> name=NC_000913:4320684..4321304 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4327959..4327339
<gbrowseImage> name=NC_007779:4327339..4327959 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

phnE(del) (Keio:JW4064)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10923[3]

phnE(del) (Keio:JW4065)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10923[3]

phnE728(del)::kan

PMID:16738554[2]

CGSC:102334

phnE729(del)::kan

PMID:16738554[2]

CGSC:102336


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4064

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCTGGCGCTGTTTATCCACAC

Primer 2:CCGATAAAGTGCTTACGCAACCG

10H5

Kohara Phage

Genobase

PMID:3038334[5]

12H2

Kohara Phage

Genobase

PMID:3038334[5]

2C12

Kohara Phage

Genobase

PMID:3038334[5]

JW4065

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCGGATGCGGTGCAAGCACC

Primer 2:CCtCTGTGGCAGCACGCCGTAGA

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[3]

est. P1 cotransduction: 59% [6]

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[3]

est. P1 cotransduction: 23% [6]
Synonyms:zjd-2231::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (EcoliWiki Page)

GeneID:948625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 5.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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