pflB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PflB

Synonyms

pyruvate formate lyase I[1], B0903[2][1], Pfl[2][1], PflB[2][1] , ECK0894, JW0886, pfl, b0903

Product description

PflB[2][3];

Component of pyruvate formate-lyase (inactive)[2][3]

Pyruvate formate lyase I; induced anaerobically; radical activated by PflA; deactivated by AdhE[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of pyruvate formate-lyase (inactive)

could be indirect

Protein

tpiA

PMID:15690043[5]

Experiment(s):EBI-882596, EBI-879853, EBI-884726

Protein

grcA

PMID:15690043[5]

Experiment(s):EBI-882596, EBI-884695, EBI-891531, EBI-890164

Protein

tpiA

PMID:16606699[6]

Experiment(s):EBI-1138311

Protein

nadE

PMID:16606699[6]

Experiment(s):EBI-1138311

Protein

matC

PMID:16606699[6]

Experiment(s):EBI-1138311

Protein

fixB

PMID:16606699[6]

Experiment(s):EBI-1138311

Protein

ydfK

PMID:16606699[6]

Experiment(s):EBI-1138311

Protein

fhuA

PMID:16606699[6]

Experiment(s):EBI-1138311

Protein

zwf

PMID:15690043[5]

Experiment(s):EBI-890164

Protein

pykA

PMID:15690043[5]

Experiment(s):EBI-890164

Protein

pykA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

ygjD

PMID:19402753[7]

MALDI(Z-score):35.025327

Protein

zwf

PMID:19402753[7]

LCMS(ID Probability):99.6

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSELNEKLAT AWEGFTKGDW QNEVNVRDFI QKNYTPYEGD ESFLAGATEA TTTLWDKVME
GVKLENRTHA PVDFDTAVAS TITSHDAGYI NKQLEKIVGL QTEAPLKRAL IPFGGIKMIE
GSCKAYNREL DPMIKKIFTE YRKTHNQGVF DVYTPDILRC RKSGVLTGLP DAYGRGRIIG
DYRRVALYGI DYLMKDKLAQ FTSLQADLEN GVNLEQTIRL REEIAEQHRA LGQMKEMAAK
YGYDISGPAT NAQEAIQWTY FGYLAAVKSQ NGAAMSFGRT STFLDVYIER DLKAGKITEQ
EAQEMVDHLV MKLRMVRFLR TPEYDELFSG DPIWATESIG GMGLDGRTLV TKNSFRFLNT
LYTMGPSPEP NMTILWSEKL PLNFKKFAAK VSIDTSSLQY ENDDLMRPDF NNDDYAIACC
VSPMIVGKQM QFFGARANLA KTMLYAINGG VDEKLKMQVG PKSEPIKGDV LNYDEVMERM
DHFMDWLAKQ YITALNIIHY MHDKYSYEAS LMALHDRDVI RTMACGIAGL SVAADSLSAI
KYAKVKPIRD EDGLAIDFEI EGEYPQFGNN DPRVDDLAVD LVERFMKKIQ KLHTYRDAIP
TQSVLTITSN VVYGKKTGNT PDGRRAGAPF GPGANPMHGR DQKGAVASLT SVAKLPFAYA
KDGISYTFSI VPNALGKDDE VRKTNLAGLM DGYFHHEASI EGGQHLNVNV MNREMLLDAM
ENPEKYPQLT IRVSGYAVRF NSLTKEQQQD VITRTFTQSM
Length

760

Mol. Wt

85.359 kDa

pI

5.9 (calculated)

Extinction coefficient

89,160 - 89,785 (calc based on 34 Y, 7 W, and 5 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P09373

Domain

630..741

PF01228 Glycine radical

PMID:19920124[8]

Domain

12..614

PF02901 Pyruvate formate lyase

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pflB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128870

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945514

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003071

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P09373

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10701

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10701

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945514

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000693

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0695

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 6.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. 7.0 7.1 7.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. 8.0 8.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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