oppA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

OppA

Synonyms

oligopeptide transporter subunit[1], periplasmic-binding component of ABC superfamily[1], B1243[2][1], OppA[2][1] , ECK1237, JW1235, b1243

Product description

OppA-oligopeptide ABC transporter substrate-binding[2][3];

Component of oligopeptide ABC transporter[2][3]

Oligopeptide transport, periplasmic oligopeptide binding protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000914

F

Seeded from EcoCyc (v14.0)

complete

GO:0006857

oligopeptide transport

PMID:3897225[5]

IDA: Inferred from Direct Assay

P

complete

GO:0015031

protein transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0653

P

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0200

C

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:3536860[6]

IDA: Inferred from Direct Assay

F

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of oligopeptide ABC transporter

could be indirect

Protein

guaA

PMID:16606699[7]

Experiment(s):EBI-1139299

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

periplasm

From EcoCyc[3]

Periplasm

PMID:9298646[8]

EchoLocation:oppA


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTNITKRSLV AAGVLAALMA GNVALAADVP AGVTLAEKQT LVRNNGSEVQ SLDPHKIEGV
PESNISRDLF EGLLVSDLDG HPAPGVAESW DNKDAKVWTF HLRKDAKWSD GTPVTAQDFV
YSWQRSVDPN TASPYASYLQ YGHIAGIDEI LEGKKPITDL GVKAIDDHTL EVTLSEPVPY
FYKLLVHPST SPVPKAAIEK FGEKWTQPGN IVTNGAYTLK DWVVNERIVL ERSPTYWNNA
KTVINQVTYL PIASEVTDVN RYRSGEIDMT NNSMPIELFQ KLKKEIPDEV HVDPYLCTYY
YEINNQKPPF NDVRVRTALK LGMDRDIIVN KVKAQGNMPA YGYTPPYTDG AKLTQPEWFG
WSQEKRNEEA KKLLAEAGYT ADKPLTINLL YNTSDLHKKL AIAASSLWKK NIGVNVKLVN
QEWKTFLDTR HQGTFDVARA GWCADYNEPT SFLNTMLSNS SMNTAHYKSP AFDSIMAETL
KVTDEAQRTA LYTKAEQQLD KDSAIVPVYY YVNARLVKPW VGGYTGKDPL DNTYTRNMYI VKH
Length

543

Mol. Wt

60.899 kDa

pI

6.5 (calculated)

Extinction coefficient

113,220 - 113,470 (calc based on 28 Y, 13 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-26

UniProt Manual:Signal Peptides

UniProt:P23843

Domain

81..464

PF00496 Bacterial extracellular solute-binding proteins, family 5 Middle

PMID:19920124[9]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=oppA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129204

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945830

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004172

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P23843

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10674

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10674

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945830

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000667

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0668

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Guyer, CA et al. (1985) Purification and characterization of a periplasmic oligopeptide binding protein from Escherichia coli. J. Biol. Chem. 260 10812-8 PubMed
  6. Guyer, CA et al. (1986) Binding specificity of the periplasmic oligopeptide-binding protein from Escherichia coli. J. Bacteriol. 168 775-9 PubMed
  7. Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  9. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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