metH:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MetH

Synonyms

homocysteine-N5-methyltetrahydrofolate transmethylase, B12-dependent[1], B4019[2][1], MetH[2][1] , ECK4011, JW3979, b4019

Product description

Methionine synthase, cobalamin-dependent; 5-methyltetrahydrofolate-homocysteine methyltransferase; binds Zn(II)[3]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004156

dihydropteroate synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000489

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004223

C

Seeded from EcoCyc (v14.0)

complete

GO:0008168

methyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0489

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc (v14.0)

complete

GO:0008705

methionine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003759

F

Seeded from EcoCyc (v14.0)

complete

GO:0008705

methionine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004223

F

Seeded from EcoCyc (v14.0)

complete

GO:0008705

methionine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011822

F

Seeded from EcoCyc (v14.0)

complete

GO:0008705

methionine synthase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.1.1.13

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:9250984[4]

IDA: Inferred from Direct Assay

C

complete

GO:0008898

homocysteine S-methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003726

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:2668277[5]

IDA: Inferred from Direct Assay

C

complete

GO:0009086

methionine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003759

P

Seeded from EcoCyc (v14.0)

complete

GO:0008705

methionine synthase activity

PMID:10978155[6]

IDA: Inferred from Direct Assay

F

complete

GO:0009086

methionine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004223

P

Seeded from EcoCyc (v14.0)

complete

GO:0008168

methyltransferase activity

Taylor and Weissbach (1971)[7]

IDA: Inferred from Direct Assay

F

Missing: reference

GO:0009086

methionine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011822

P

Seeded from EcoCyc (v14.0)

complete

GO:0008898

homocysteine S-methyltransferase activity

Taylor and Weissbach (1971)[7]

IDA: Inferred from Direct Assay

F

Missing: reference

GO:0009086

methionine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0486

P

Seeded from EcoCyc (v14.0)

complete

GO:0009257

10-formyltetrahydrofolate biosynthetic process

Taylor and Weissbach (1971)[7]

IDA: Inferred from Direct Assay

P

Missing: reference

GO:0009396

folic acid and derivative biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000489

P

Seeded from EcoCyc (v14.0)

complete

GO:0008705

methionine synthase activity

Taylor and Weissbach (1971)[7]

IDA: Inferred from Direct Assay

F

Missing: reference

GO:0031419

cobalamin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003759

F

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

Taylor and Weissbach (1971)[7]

IDA: Inferred from Direct Assay

P

Missing: reference

GO:0031419

cobalamin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006158

F

Seeded from EcoCyc (v14.0)

complete

GO:0009086

methionine biosynthetic process

Taylor and Weissbach (1971)[7]

IDA: Inferred from Direct Assay

P

Missing: reference

GO:0031419

cobalamin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011822

F

Seeded from EcoCyc (v14.0)

complete

GO:0031419

cobalamin binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0846

F

Seeded from EcoCyc (v14.0)

complete

GO:0044237

cellular metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011005

P

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003759

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006158

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0050897

cobalt ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011822

F

Seeded from EcoCyc (v14.0)

complete

GO:0050897

cobalt ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0170

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

gltB

PMID:16606699[8]

Experiment(s):EBI-1147304

Protein

dnaE

PMID:16606699[8]

Experiment(s):EBI-1147304

Protein

ilvC

PMID:16606699[8]

Experiment(s):EBI-1147304

Protein

bglX

PMID:16606699[8]

Experiment(s):EBI-1147304

Small Molecule

Cobalamin

PMID:9201956[9]


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSSKVEQLRA QLNERILVLD GGMGTMIQSY RLNEADFRGE RFADWPCDLK GNNDLLVLSK
PEVIAAIHNA YFEAGADIIE TNTFNSTTIA MADYQMESLS AEINFAAAKL ARACADEWTA
RTPEKPRYVA GVLGPTNRTA SISPDVNDPA FRNITFDGLV AAYRESTKAL VEGGADLILI
ETVFDTLNAK AAVFAVKTEF EALGVELPIM ISGTITDASG RTLSGQTTEA FYNSLRHAEA
LTFGLNCALG PDELRQYVQE LSRIAECYVT AHPNAGLPNA FGEYDLDADT MAKQIREWAQ
AGFLNIVGGC CGTTPQHIAA MSRAVEGLAP RKLPEIPVAC RLSGLEPLNI GEDSLFVNVG
ERTNVTGSAK FKRLIKEEKY SEALDVARQQ VENGAQIIDI NMDEGMLDAE AAMVRFLNLI
AGEPDIARVP IMIDSSKWDV IEKGLKCIQG KGIVNSISMK EGVDAFIHHA KLLRRYGAAV
VVMAFDEQGQ ADTRARKIEI CRRAYKILTE EVGFPPEDII FDPNIFAVAT GIEEHNNYAQ
DFIGACEDIK RELPHALISG GVSNVSFSFR GNDPVREAIH AVFLYYAIRN GMDMGIVNAG
QLAIYDDLPA ELRDAVEDVI LNRRDDGTER LLELAEKYRG SKTDDTANAQ QAEWRSWEVN
KRLEYSLVKG ITEFIEQDTE EARQQATRPI EVIEGPLMDG MNVVGDLFGE GKMFLPQVVK
SARVMKQAVA YLEPFIEASK EQGKTNGKMV IATVKGDVHD IGKNIVGVVL QCNNYEIVDL
GVMVPAEKIL RTAKEVNADL IGLSGLITPS LDEMVNVAKE MERQGFTIPL LIGGATTSKA
HTAVKIEQNY SGPTVYVQNA SRTVGVVAAL LSDTQRDDFV ARTRKEYETV RIQHGRKKPR
TPPVTLEAAR DNDFAFDWQA YTPPVAHRLG VQEVEASIET LRNYIDWTPF FMTWSLAGKY
PRILEDEVVG VEAQRLFKDA NDMLDKLSAE KTLNPRGVVG LFPANRVGDD IEIYRDETRT
HVINVSHHLR QQTEKTGFAN YCLADFVAPK LSGKADYIGA FAVTGGLEED ALADAFEAQH
DDYNKIMVKA LADRLAEAFA EYLHERVRKV YWGYAPNENL SNEELIRENY QGIRPAPGYP
ACPEHTEKAT IWELLEVEKH TGMKLTESFA MWPGASVSGW YFSHPDSKYY AVAQIQRDQV
EDYARRKGMS VTEVERWLAP NLGYDAD
Length

1,227

Mol. Wt

135.998 kDa

pI

4.8 (calculated)

Extinction coefficient

136,600 - 138,225 (calc based on 40 Y, 14 W, and 13 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P13009

Domain

747..851

PF02310 B12 binding domain

PMID:19920124[10]

Domain

657..740

PF02607 B12 binding domain

PMID:19920124[10]

Domain

1055..1192

PF02965 Vitamin B12 dependent methionine synthase, activation domain

PMID:19920124[10]

Domain

356..568

PF00809 Pterin binding enzyme

PMID:19920124[10]

Domain

16..327

PF02574 Homocysteine S-methyltransferase

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=metH taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131845

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948522

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013140

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P13009

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10587

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10587

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948522

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000580

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0582

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  4. ↑ Jarrett, JT et al. (1997) Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli. Meth. Enzymol. 281 196-213 PubMed
  5. ↑ Banerjee, RV et al. (1989) Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain. J. Biol. Chem. 264 13888-95 PubMed
  6. ↑ Hall, DA et al. (2000) Interaction of flavodoxin with cobalamin-dependent methionine synthase. Biochemistry 39 10711-9 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Taylor, R.T. and Weissbach, H. NS-methyltetrahydrofolate-homocysteine (Vitamin B12) methyltransferase (Escherichia coli B). Methods Enzymol. 17B (1971) 379-388.Taylor & Weissbach Cite error: Invalid <ref> tag; name "Taylor" defined multiple times with different content Cite error: Invalid <ref> tag; name "Taylor" defined multiple times with different content Cite error: Invalid <ref> tag; name "Taylor" defined multiple times with different content Cite error: Invalid <ref> tag; name "Taylor" defined multiple times with different content Cite error: Invalid <ref> tag; name "Taylor" defined multiple times with different content
  8. ↑ 8.0 8.1 8.2 8.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ Goulding, CW et al. (1997) Cobalamin-dependent methionine synthase is a modular protein with distinct regions for binding homocysteine, methyltetrahydrofolate, cobalamin, and adenosylmethionine. Biochemistry 36 8082-91 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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