metF:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

metF

Mnemonic

Methionine

Synonyms

ECK3933, b3941, JW3913[1], JW3913

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

89.03 minutes, 89.03 minutes 

MG1655: 4130639..4131529
<gbrowseImage> name=NC_000913:4130639..4131529 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4112723..4113613
<gbrowseImage> name=NC_012967:4112723..4113613 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4020308..4021198
<gbrowseImage> name=NC_012759:4020308..4021198 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3504065..3503175
<gbrowseImage> name=NC_007779:3503175..3504065 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4230336..4231226
<gbrowseImage> name=NC_010473:4230336..4231226 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmetF (Keio:JW3913)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10826[3]

metF63

CGSC:5456

metF27

CGSC:8385

metF24

PMID:14047235[4]

CGSC:9070

metF159(Am)

CGSC:9687

amber (UAG) mutation

metF178

CGSC:12210

metF64

CGSC:13851

ΔmetF728::kan

PMID:16738554[2]

CGSC:101832


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3913

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGCTTTTTTCACGCCAGCCA

Primer 2:CCTAAACCAGGTCGAACCCCCAG

6D8

Kohara Phage

Genobase

PMID:3038334[6]

12E3

Kohara Phage

Genobase

PMID:3038334[6]

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[3]

est. P1 cotransduction: 2% [7]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[3]

est. P1 cotransduction: 65% [7]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10585

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10585

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000578

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948432

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0580

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012897

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. PITTARD, J et al. (1963) GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J. Bacteriol. 85 1394-401 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories