mcrA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mcrA

Mnemonic

Modified cytosine restriction

Synonyms

ECK1145, b1159, JW1145, rglA[1], rglA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

26.07 minutes 

MG1655: 1209569..1210402
<gbrowseImage> name=NC_000913:1209569..1210402 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1211923..1212756
<gbrowseImage> name=NC_007779:1211923..1212756 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmcrA (Keio:JW1145)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9065[3]

mcrA0

CGSC:10892

ΔmcrA747::kan

PMID:16738554[2]

CGSC:101770


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Notes

Raleigh et al.[4] tested the mcrA and mcrB phenotypes of 94 E. coli strains.


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1145

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCATGTTTTTGATAATAATGG

Primer 2:CCTTTCTGTAATCGGTTTATATT

3E11

Kohara Phage

Genobase

PMID:3038334[6]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[3]

est. P1 cotransduction: 7% [7]

zcf-117::Tn10

Linked marker

CAG18463 = CGSC7363[3]

est. P1 cotransduction: 96% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10573

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10573

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000566

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945727

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0568

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003891

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Raleigh, EA et al. (1988) McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 16 1563-75 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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