lysT:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lysT

Mnemonic

Lysine

Synonyms

ECK0732, b0743, JWR0018, Su(beta), lysT-alpha, su-5, supG, supK, supL, lysTalpha, sua, Su[be, Su[beta], alpha[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

16.81 minutes 

MG1655: 779777..779852
<gbrowseImage> name=NC_000913:779777..779852 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 761436..761511
<gbrowseImage> name=NC_012967:761436..761511 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 682537..682612
<gbrowseImage> name=NC_012759:682537..682612 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 780976..781051
<gbrowseImage> name=NC_007779:780976..781051 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 832369..832444
<gbrowseImage> name=NC_010473:832369..832444 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lysT102(OS)

ochre (UAA) suppressor

CGSC:11285

lysT76(OS)

ochre (UAA) suppressor

PMID:6207301[3]

CGSC:8594

lysT46(OS)

ochre (UAA) suppressor

CGSC:11605


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[4]

Status:

Primer 1:

Primer 2:

10G5

Kohara Phage

Genobase

PMID:3038334[5]

4H1

Kohara Phage

Genobase

PMID:3038334[5]

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[6]

est. P1 cotransduction: 99% [7]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[6]

est. P1 cotransduction: 17% [7]
Synonyms:zbi-29::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG30055

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG30055

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002497

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945336

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4218

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002525

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Yoshimura, M et al. (1984) Identification of transfer RNA suppressors in Escherichia coli. III. Ochre suppressors of lysine tRNA. J. Mol. Biol. 177 609-25 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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