lysA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lysA

Mnemonic

Lysine

Synonyms

ECK2836, b2838, JW2806[1], JW2806

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

64.14 minutes 

MG1655: 2976921..2975659
<gbrowseImage> name=NC_000913:2975659..2976921 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2873520..2872258
<gbrowseImage> name=NC_012967:2872258..2873520 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2862807..2864069
<gbrowseImage> name=NC_012759:2862807..2864069 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2977555..2976293
<gbrowseImage> name=NC_007779:2976293..2977555 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3070791..3069529
<gbrowseImage> name=NC_010473:3069529..3070791 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔlysA (Keio:JW2806)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10193[3]

lysA23

CGSC:5087

lysA29

CGSC:5241

lysA22

CGSC:6775

lysA24

CGSC:9365

lysA0::Tn10

CGSC:10967

lysA27::Mu

CGSC:11575

lysA35

CGSC:12208

lysA28

CGSC:14251

lysA25

CGSC:72557

ΔlysA763::kan

PMID:16738554[2]

CGSC:101706


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2806

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCACATTCACTGTTCAGCAC

Primer 2:CCAAGCAATTCCAGCGCCAGTAA

8H3

Kohara Phage

Genobase

PMID:3038334[5]

12H3

Kohara Phage

Genobase

PMID:3038334[5]

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[3]

est. P1 cotransduction: 39% [6]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[3]

est. P1 cotransduction: % [6]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10549

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10549

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000542

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947313

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0544

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009309

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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