leuS:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

leuS

Mnemonic

Leucine

Synonyms

ECK0635, b0642, JW0637, syl[1]

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

14.47 minutes 

MG1655: 674006..671424
<gbrowseImage> name=NC_000913:671424..674006 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 657367..654785
<gbrowseImage> name=NC_012967:654785..657367 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 574184..576766
<gbrowseImage> name=NC_012759:574184..576766 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 675205..672623
<gbrowseImage> name=NC_007779:672623..675205 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 613338..610756
<gbrowseImage> name=NC_010473:610756..613338 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 726598..724016
<gbrowseImage> name=NC_010473:724016..726598 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

leuS31(ts)

temperature sensitive

CGSC:9557


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0637

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCCAAGAGCAATACCGCCCGGA

Primer 2:CCGCCAACGACCAGATTGAGGAG

15D7

Kohara Phage

Genobase

PMID:3038334[3]

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[4]

est. P1 cotransduction: 54% [5]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[4]

est. P1 cotransduction: 32% [5]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10532

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10532

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000525

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947497

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0527

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002196

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories