lepA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LepA

Synonyms

GTP-binding membrane protein[1], B2569[2][1], LepA[2][1], EF4[3][1] , ECK2567, JW2553, b2569

Product description

GTP-binding membrane protein[2]; elongation factor 4[3]

Back-translocating Elongation Factor EF4, GTPase; required for efficient and accurate protein synthesis[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00071

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000640

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004161

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005225

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006297

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0039

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0200

C

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

argA

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

macA

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

zntB

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

hyfE

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

recE

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rplD

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rplI

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rplM

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rplV

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rplX

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rpsB

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rpsE

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rpsF

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rpsG

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

rpsJ

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

puuP

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

yeiN

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

yidX

PMID:15690043[5]

Experiment(s):EBI-893163

Protein

hyfE

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

macA

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

recE

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

puuP

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

yidX

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

psuG

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

argA

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

ydaN

PMID:19402753[6]

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER GITIKAQSVT
LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM
EMDLEVVPVL NKIDLPAADP ERVAEEIEDI VGIDATDAVR CSAKTGVGVQ DVLERLVRDI
PPPEGDPEGP LQALIIDSWF DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF
TPKQVDRTEL KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF
PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI IQERLEREYD
LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR EPIAECHMLL PQAYLGNVIT
LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQASDM
VRVDVLINGE RVDALALITH RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS
TVKQLRKNVL AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK
Length

599

Mol. Wt

66.571 kDa

pI

5.4 (calculated)

Extinction coefficient

39,310 - 40,560 (calc based on 19 Y, 2 W, and 10 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

398..486

PF00679 Elongation factor G C-terminus

PMID:19920124[7]

Domain

2..182

PF00009 Elongation factor Tu GTP binding domain

PMID:19920124[7]

Domain

205..275

PF03144 Elongation factor Tu domain 2

PMID:19920124[7]

Domain

487..595

PF06421 GTP-binding protein LepA C-terminus

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lepA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130494

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947051

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008452

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P60785

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10529

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10529

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947051

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000522

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0524

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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