kdsA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

KdsA

Synonyms

3-deoxy-D-manno-octulosonate 8-phosphate synthase[1], B1215[2][1], KdsA[2][1] , ECK1203, JW1206, b1215

Product description

KdsA[2]; 3-deoxy-D-manno-octulosonate 8-phosphate synthase[3];

Component of [[:Category:Complex:3-deoxy-D-manno-octulosonic acid 8-phosphate synthase|3-deoxy-D-manno-octulosonic acid 8-phosphate synthase]][2]; [[:Category:Complex:3-deoxy-D-manno-octulosonic acid 8-phosphate synthase|3-deoxy-D-manno-octulosonate 8-phosphate synthase]][3]

3-deoxy-D-manno-octulosonate 8-phosphate (KDO8-P) synthase; LPS biosynthesis[4]

EC number (for enzymes)

<protect></protect>

Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005829

cytosol

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0008676

3-deoxy-8-phosphooctulonate synthase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00056

F

Seeded from EcoCyc (v14.0)

complete

GO:0008676

3-deoxy-8-phosphooctulonate synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006269

F

Seeded from EcoCyc (v14.0)

complete

GO:0008676

3-deoxy-8-phosphooctulonate synthase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.5.1.55

F

Seeded from EcoCyc (v14.0)

complete

GO:0019294

keto-3-deoxy-D-manno-octulosonic acid biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00056

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of [[:Category:Complex:3-deoxy-D-manno-octulosonic acid 8-phosphate synthase|3-deoxy-D-manno-octulosonic acid 8-phosphate synthase]]

could be indirect

Protein

djlA

PMID:16606699[6]

Experiment(s):EBI-1139204

Protein

mdoD

PMID:16606699[6]

Experiment(s):EBI-1139204

Protein

groL

PMID:16606699[6]

Experiment(s):EBI-1139204

Protein

sdhA

PMID:16606699[6]

Experiment(s):EBI-1139204

Protein

groL

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

rcsC

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

hldD

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

rplA

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

rplS

PMID:15690043[7]

Experiment(s):EBI-881294, EBI-888757

Protein

rplV

PMID:15690043[7]

Experiment(s):EBI-881294, EBI-888757

Protein

rplX

PMID:15690043[7]

Experiment(s):EBI-881294, EBI-888757

Protein

rpsC

PMID:15690043[7]

Experiment(s):EBI-881294, EBI-888757

Protein

rpsM

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

tufA

PMID:15690043[7]

Experiment(s):EBI-881294, EBI-888757

Protein

ybdN

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

gyrA

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

kdsB

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

metK

PMID:15690043[7]

Experiment(s):EBI-881294

Protein

ypeA

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

hupA

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

murE

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplC

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplD

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplE

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplF

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplI

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplL

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplM

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplN

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplY

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpmB

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpmG

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsB

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsD

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsE

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsG

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsI

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsJ

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsK

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsN

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsT

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

yagG

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

yfiF

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

yihR

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rplU

PMID:15690043[7]

Experiment(s):EBI-888757

Protein

rpsN

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplE

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753[8]

LCMS(ID Probability):99.0 MALDI(Z-score):12.154008

Protein

rpsI

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsM

PMID:19402753[8]

MALDI(Z-score):19.109181

Protein

rplU

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753[8]

LCMS(ID Probability):99.0 MALDI(Z-score):15.027321

Protein

gyrA

PMID:19402753[8]

MALDI(Z-score):20.708604

Protein

rpsC

PMID:19402753[8]

LCMS(ID Probability):99.0 MALDI(Z-score):19.388132

Protein

hupA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplA

PMID:19402753[8]

MALDI(Z-score):18.777277

Protein

rfaD

PMID:19402753[8]

MALDI(Z-score):33.513134

Protein

ypeA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

murE

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

malP

PMID:19402753[8]

MALDI(Z-score):21.120618

Protein

lon

PMID:19402753[8]

MALDI(Z-score):24.937062

Protein

rplD

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):19.721490

Protein

ycbZ

PMID:19402753[8]

MALDI(Z-score):26.982999

Protein

yagG

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

yihR

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

rplL

PMID:19402753[8]

LCMS(ID Probability):99.6

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


<protect></protect>

Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKQKVVSIGD INVANDLPFV LFGGMNVLES RDLAMRICEH YVTVTQKLGI PYVFKASFDK
ANRSSIHSYR GPGLEEGMKI FQELKQTFGV KIITDVHEPS QAQPVADVVD VIQLPAFLAR
QTDLVEAMAK TGAVINVKKP QFVSPGQMGN IVDKFKEGGN EKVILCDRGA NFGYDNLVVD
MLGFSIMKKV SGNSPVIFDV THALQCRDPF GAASGGRRAQ VAELARAGMA VGLAGLFIEA
HPDPEHAKCD GPSALPLAKL EPFLKQMKAI DDLVKGFEEL DTSK
Length

284

Mol. Wt

30.832 kDa

pI

6.9 (calculated)

Extinction coefficient

5,960 - 6,460 (calc based on 4 Y, 0 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

2..276

PF00793 DAHP synthetase I family

PMID:19920124[9]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=kdsA taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129178

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945785

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004077

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A715

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10518

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10518

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945785

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000511

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0513

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. 6.0 6.1 6.2 6.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 7.23 7.24 7.25 7.26 7.27 7.28 7.29 7.30 7.31 7.32 7.33 7.34 7.35 7.36 7.37 7.38 7.39 7.40 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 8.16 8.17 8.18 8.19 8.20 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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