hemN:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
hemN |
|---|---|
| Mnemonic |
Hemin |
| Synonyms |
ECK3860, b3867, JW3838, yihJ[1], yihJ |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
87.29 minutes |
MG1655: 4050068..4051441 |
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NC_012967: 4030342..4031715 |
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NC_012759: 3939737..3941110 |
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W3110 |
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W3110: 3584636..3583263 |
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DH10B: 4148988..4150361 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔhemN (Keio:JW3838) |
deletion |
deletion |
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hemNH58L |
H58L |
Results in loss of iron-sulfur cluster and activity |
seeded from UniProt:P32131 | ||||
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hemNC62S |
C62S |
Results in loss of iron-sulfur cluster and activity |
seeded from UniProt:P32131 | ||||
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hemNC66S |
C66S |
Results in loss of iron-sulfur cluster and activity |
seeded from UniProt:P32131 | ||||
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hemNF68L |
F68L |
No effect |
seeded from UniProt:P32131 | ||||
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hemNC69S |
C69S |
Results in loss of iron-sulfur cluster and activity |
seeded from UniProt:P32131 | ||||
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hemNC71S |
C71S |
No effect on iron-sulfur cluster, but results in activity loss |
seeded from UniProt:P32131 | ||||
|
hemNG111V |
G111V |
Loss of activity and much less iron-sulfur cluster formed; when associated with V-113 |
seeded from UniProt:P32131 | ||||
|
hemNG113V |
G113V |
Loss of activity and much less iron-sulfur cluster formed; when associated with V-111 |
seeded from UniProt:P32131 | ||||
|
hemNE145A,I |
E145A,I |
Loss of activity. Iron content reduced by about 80% |
seeded from UniProt:P32131 | ||||
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hemNF310A,L |
F310A,L |
Loss of activity. Iron content reduced by about 50%. Can cleave up to one molecule of S-adenosyl-L-methionine (in vitro) |
seeded from UniProt:P32131 | ||||
|
hemNQ311A |
Q311A |
Loss of activity. No change in iron content. Can cleave up to one molecule of S-adenosyl-L-methionine (in vitro) |
seeded from UniProt:P32131 | ||||
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hemNI329A |
I329A |
Loss of activity. No change in iron content. Can cleave up to one molecule of S-adenosyl-L-methionine (in vitro) |
seeded from UniProt:P32131 | ||||
|
hemNY56A,L |
Y56A,L |
Loss of activity |
seeded from UniProt:P32131 | ||||
|
hemNY56F |
Y56F |
Decreases activity by 50% |
seeded from UniProt:P32131 | ||||
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ΔhemN728::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3838 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCTGTACAGCAAATCGACTG Primer 2:CCAATCACCCGAGAGAACTGCTG | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 43% [6] | ||
|
Linked marker |
est. P1 cotransduction: 61% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 5.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
