groL:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

GroEL

Synonyms

Cpn60 chaperonin GroEL, large subunit of GroESL[1], B4143[2][1], Hdh[2][1], TabB[2][1], GroL[2][1], MopA[2][1], GroEL[2][1] , ECK4137, groEL, JW4103, mopA, b4143

Product description

GroEL, chaperone Hsp60, peptide-dependent ATPase, heat shock protein[2][3];

Component of GroEL-GroES Chaperonin-CPLX[3]

Chaperonin Cpn60; phage morphogenesis; GroESL large subunit GroEL, weak ATPase; binds Ap4A[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0042963

phage assembly

PMID:7015340[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0016887

ATPase activity

PMID:379350[6]

IDA: Inferred from Direct Assay

F

complete

GO:0009408

response to heat

PMID:8349564[7]

IEP: Inferred from Expression Pattern

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of GroEL-GroES Chaperonin-CPLX

could be indirect

Protein

aceE

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

clpB

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

lon

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

narG

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

rpsB

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

ybbN

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

yibA

PMID:15690043[8]

Experiment(s):EBI-885524

Protein

nuoG

PMID:16606699[9]

Experiment(s):EBI-1147548

Protein

rhsA

PMID:16606699[9]

Experiment(s):EBI-1147548

Protein

yjeS

PMID:16606699[9]

Experiment(s):EBI-1147597

Protein

dnaK

PMID:15690043[8]

Experiment(s):EBI-892151

Protein

grpE

PMID:15690043[8]

Experiment(s):EBI-892151

Protein

pyrD

PMID:15690043[8]

Experiment(s):EBI-892151

Protein

ycgR

PMID:15690043[8]

Experiment(s):EBI-892151

Protein

yhdJ

PMID:15690043[8]

Experiment(s):EBI-892151

Protein

grpE

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

ybbN

PMID:19402753[10]

MALDI(Z-score):17.053160

Protein

ycgR

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

pyrD

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

yhdJ

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

gpB of bacteriophage lambda


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Notes

In vivo substrates for GroEL have been identified by proteomic analysis. The properties of these GroEL substrates has been reviewed [11]

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI
ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI
DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG
TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV
AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV
ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY
DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI
RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA
ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG
MGGMGGMM
Length

548

Mol. Wt

57.328 kDa

pI

4.7 (calculated)

Extinction coefficient

10,430 - 10,805 (calc based on 7 Y, 0 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6F5

Domain

23..525

PF00118 TCP-1/cpn60 chaperonin family

PMID:19920124[12]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=groL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

See Help:Jmol in EcoliWiki

Text label
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >1AON.pdb</uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>









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Notes

The automated system for homologous structures in the pdb selects a fragment structure. The entry 1AON, which is a more complete structure, is shown in the structure viewer.

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131968

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948665

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013568

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6F5

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10599

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10599

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948665

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000592

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0594

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Tilly, K et al. (1981) Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis. Proc. Natl. Acad. Sci. U.S.A. 78 1629-33 PubMed
  6. ↑ Hendrix, RW (1979) Purification and properties of groE, a host protein involved in bacteriophage assembly. J. Mol. Biol. 129 375-92 PubMed
  7. ↑ Chuang, SE & Blattner, FR (1993) Characterization of twenty-six new heat shock genes of Escherichia coli. J. Bacteriol. 175 5242-52 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. ↑ 9.0 9.1 9.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  11. ↑ Ellis, RJ (2005) Chaperomics: in vivo GroEL function defined. Curr. Biol. 15 R661-3 PubMed
  12. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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