fusA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FusA

Synonyms

protein chain elongation factor EF-G, GTP-binding[1], B3340[2][1], Fus[2][1], Far[2][1], FusA[2][1], EF-G[2][1] , ECK3327, far, fus, JW3302, b3340

Product description

elongation factor G[2][3]

Elongation Factor EF-G; GTPase required for translocation from the A-site to the P-site in the ribosome; fusidic acid resistance[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003746

translation elongation factor activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00054

F

Seeded from EcoCyc (v14.0)

complete

GO:0003746

translation elongation factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004540

F

Seeded from EcoCyc (v14.0)

complete

GO:0003746

translation elongation factor activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0251

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00054

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000640

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004161

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004540

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005225

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005517

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00054

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006414

translational elongation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00054

P

Seeded from EcoCyc (v14.0)

complete

GO:0006414

translational elongation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004540

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

nadE

PMID:15690043[5]

Experiment(s):EBI-887996

Protein

tufA

PMID:15690043[5]

Experiment(s):EBI-887996

Protein

apt

PMID:15690043[5]

Experiment(s):EBI-887996

Protein

aroB

PMID:15690043[5]

Experiment(s):EBI-887996

Protein

ydiV

PMID:15690043[5]

Experiment(s):EBI-893622

Protein

ypdI

PMID:15690043[5]

Experiment(s):EBI-893622

Protein

citE

PMID:15690043[5]

Experiment(s):EBI-893622

Protein

htrE

PMID:15690043[5]

Experiment(s):EBI-893622

Protein

rmlA2

PMID:15690043[5]

Experiment(s):EBI-893622

Protein

yfcC

PMID:15690043[5]

Experiment(s):EBI-893622

Protein

rhsA

PMID:16606699[6]

Experiment(s):EBI-1145597

Protein

yeaG

PMID:16606699[6]

Experiment(s):EBI-1145597

Protein

rbsA

PMID:16606699[6]

Experiment(s):EBI-1145597

Protein

ybjD

PMID:16606699[6]

Experiment(s):EBI-1145597

Protein

murF

PMID:19402753[7]

MALDI(Z-score):37.906501

Protein

murG

PMID:19402753[7]

MALDI(Z-score):37.396251

Protein

nadE

PMID:19402753[7]

MALDI(Z-score):39.983110

Protein

htrE

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

murI

PMID:19402753[7]

MALDI(Z-score):38.761999

Protein

aroB

PMID:19402753[7]

MALDI(Z-score):38.906883

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytoplasm

PMID:7042386[8]

EchoLocation:fusA

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MARTTPIARY RNIGISAHID AGKTTTTERI LFYTGVNHKI GEVHDGAATM DWMEQEQERG
ITITSAATTA FWSGMAKQYE PHRINIIDTP GHVDFTIEVE RSMRVLDGAV MVYCAVGGVQ
PQSETVWRQA NKYKVPRIAF VNKMDRMGAN FLKVVNQIKT RLGANPVPLQ LAIGAEEHFT
GVVDLVKMKA INWNDADQGV TFEYEDIPAD MVELANEWHQ NLIESAAEAS EELMEKYLGG
EELTEAEIKG ALRQRVLNNE IILVTCGSAF KNKGVQAMLD AVIDYLPSPV DVPAINGILD
DGKDTPAERH ASDDEPFSAL AFKIATDPFV GNLTFFRVYS GVVNSGDTVL NSVKAARERF
GRIVQMHANK REEIKEVRAG DIAAAIGLKD VTTGDTLCDP DAPIILERME FPEPVISIAV
EPKTKADQEK MGLALGRLAK EDPSFRVWTD EESNQTIIAG MGELHLDIIV DRMKREFNVE
ANVGKPQVAY RETIRQKVTD VEGKHAKQSG GRGQYGHVVI DMYPLEPGSN PKGYEFINDI
KGGVIPGEYI PAVDKGIQEQ LKAGPLAGYP VVDMGIRLHF GSYHDVDSSE LAFKLAASIA
FKEGFKKAKP VLLEPIMKVE VETPEENTGD VIGDLSRRRG MLKGQESEVT GVKIHAEVPL
SEMFGYATQL RSLTKGRASY TMEFLKYDEA PSNVAQAVIE ARGK
Length

704

Mol. Wt

77.581 kDa

pI

5.2 (calculated)

Extinction coefficient

61,310 - 61,685 (calc based on 19 Y, 6 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6M8

Modification Site

692

phosphorylation site at S692

probability greater than 75%

PMID:17938405[9]

Modification Site

636

phosphorylation site at S636

probability greater than 75%

PMID:17938405[9]

Domain

611..698

PF00679 Elongation factor G C-terminus

PMID:19920124[10]

Domain

486..609

PF03764 Elongation factor G, domain IV

PMID:19920124[10]

Domain

8..288

PF00009 Elongation factor Tu GTP binding domain

PMID:19920124[10]

Domain

331..398

PF03144 Elongation factor Tu domain 2

PMID:19920124[10]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fusA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131219

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947847

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010914

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6M8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10360

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10360

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947847

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000353

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0355

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ Ovchinnikov YuA, et al. (1982) The primary structure of elongation factor G from Escherichia coli. A complete amino acid sequence. FEBS Lett. 139 130-5 PubMed
  9. ↑ 9.0 9.1 Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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