erpA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
erpA |
|---|---|
| Mnemonic |
Essential Respiratory Protein |
| Synonyms |
ECK0155, b0156, JW0152, yadR[1], JW0152, yadR |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
3.81 minutes |
MG1655: 176610..176954 |
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NC_012759: 176609..176953 |
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W3110 |
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W3110: 176610..176954 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
erpAC108S |
C108S |
Does not complement knockout |
seeded from UniProt:P0ACC3 | ||||
|
erpAC42S |
C42S |
Does not complement knockout |
seeded from UniProt:P0ACC3 | ||||
|
erpAC106S |
C106S |
Does not complement knockout |
seeded from UniProt:P0ACC3 | ||||
|
erpA(del) |
Deletion |
Deletion |
Auxotrophies |
erpA is an ATC protein (A-type carrier protein) involved in the maturation of the nitrate-indicuble, multisubunit anaerobix respiratory enzymes Formate dehydrogenase-N (Fdh-N) and nitrate reductase (Nar). Deletion of erpA inactivated both Fdh-N and Nar enzymes, Table 2. |
Parent strain: MC4100 Experimental strain: LL402 |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0152 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGTGATGACGTAGCACTGCC Primer 2:CCGATACTAAAGGAAGAACCGCA | |
|
Linked marker |
est. P1 cotransduction: 34% [5] | ||
|
Linked marker |
est. P1 cotransduction: 7% [5] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Pinske, C & Sawers, RG (2012) A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12. J. Bacteriol. 194 346-53 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
