entF:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

EntF

Synonyms

enterobactin synthase multienzyme complex component, ATP-dependent[1], B0586 [2][1], holo-serine activating enzyme [2][1],apo-serine activating enzyme[2][1], ECK0579, JW0578, b0586

Product description

ENTF-PANT[2][3];

Component of enterobactin synthase multienzyme complex[2][3]

Enterochelin synthase, component F; multifunctional: holo-EntF(PCP), primed by EntD; seryl-AMP, seryl-S-EntF synthase; DHB-Ser-S-EntF intermediate formation; chain-releasing thioesterase (TE) domain is both a cyclotrimerizing lactone synthase and an elong[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000036

acyl carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009081

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000873

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0511

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006811

ion transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0406

P

Seeded from EcoCyc (v14.0)

complete

GO:0006826

iron ion transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0410

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000873

P

Seeded from EcoCyc (v14.0)

complete

GO:0009058

biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001031

P

Seeded from EcoCyc (v14.0)

complete

GO:0009239

enterobactin biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0259

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016788

hydrolase activity, acting on ester bonds

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001031

F

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010071

F

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc (v14.0)

complete

GO:0031177

phosphopantetheine binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006162

F

Seeded from EcoCyc (v14.0)

complete

GO:0048037

cofactor binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006163

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of enterobactin synthase multienzyme complex

could be indirect

Protein

ndh

PMID:16606699[5]

Experiment(s):EBI-1137240

Protein

carA

PMID:16606699[5]

Experiment(s):EBI-1137240

Protein

tatE

PMID:16606699[5]

Experiment(s):EBI-1137240

Protein

groL

PMID:16606699[5]

Experiment(s):EBI-1137240

Protein

ycgX

PMID:16606699[5]

Experiment(s):EBI-1137240
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSQHLPLVAA QPGIWMAEKL SELPSAWSVA HYVELTGEVD SPLLARAVVA GLAQADTLRM
RFTEDNGEVW QWVDDALTFE LPEIIDLRTN IDPHGTAQAL MQADLQQDLR VDSGKPLVFH
QLIQVADNRW YWYQRYHHLL VDGFSFPAIT RQIANIYCTW LRGEPTPASP FTPFADVVEE
YQQYRESEAW QRDAAFWAEQ RRQLPPPASL SPAPLPGRSA SADILRLKLE FTDGEFRQLA
TQLSGVQRTD LALALAALWL GRLCNRMDYA AGFIFMRRLG SAALTATGPV LNVLPLGIHI
AAQETLPELA TRLAAQLKKM RRHQRYDAEQ IVRDSGRAAG DEPLFGPVLN IKVFDYQLDI
PDVQAQTHTL ATGPVNDLEL ALFPDVHGDL SIEILANKQR YDEPTLIQHA ERLKMLIAQF
AADPALLCGD VDIMLPGEYA QLAQLNATQV EIPETTLSAL VAEQAAKTPD APALADARYL
FSYREMREQV VALANLLRER GVKPGDSVAV ALPRSVFLTL ALHAIVEAGA AWLPLDTGYP
DDRLKMMLED ARPSLLITTD DQLPRFSDVP NLTSLCYNAP LTPQGSAPLQ LSQPHHTAYI
IFTSGSTGRP KGVMVGQTAI VNRLLWMQNH YPLTGEDVVA QKTPCSFDVS VWEFFWPFIA
GAKLVMAEPE AHRDPLAMQQ FFAEYGVTTT HFVPSMLAAF VASLTPQTAR QSCATLKQVF
CSGEALPADL CREWQQLTGA PLHNLYGPTE AAVDVSWYPA FGEELAQVRG SSVPIGYPVW
NTGLRILDAM MHPVPPGVAG DLYLTGIQLA QGYLGRPDLT ASRFIADPFA PGERMYRTGD
VARWLDNGAV EYLGRSDDQL KIRGQRIELG EIDRVMQALP DVEQAVTHAC VINQAAATGG
DARQLVGYLV SQSGLPLDTS ALQAQLRETL PPHMVPVVLL QLPQLPLSAN GKLDRKALPL
PELKAQAPGR APKAGSETII AAAFSSLLGC DVQDADADFF ALGGHSLLAM KLAAQLSRQV
ARQVTPGQVM VASTVAKLAT IIDAEEDSTR RMGFETILPL REGNGPTLFC FHPASGFAWQ
FSVLSRYLDP QWSIIGIQSP RPNGPMQTAA NLDEVCEAHL ATLLEQQPHG PYYLLGYSLG
GTLAQGIAAR LRARGEQVAF LGLLDTWPPE TQNWQEKEAN GLDPEVLAEI NREREAFLAA
QQGSTSTELF TTIEGNYADA VRLLTTAHSV PFDGKATLFV AERTLQEGMS PERAWSPWIA
ELDIYRQDCA HVDIISPGTF EKIGPIIRAT LNR
Length

1,293

Mol. Wt

141.993 kDa

pI

4.9 (calculated)

Extinction coefficient

181,170 - 182,795 (calc based on 33 Y, 24 W, and 13 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

482..887

PF00501 AMP-binding enzyme

PMID:19920124[6]

Domain

1..301

PF00668 Condensation domain

PMID:19920124[6]

Domain

978..1042

PF00550 Phosphopantetheine attachment site

PMID:19920124[6]

Domain

1066..1290

PF00975 Thioesterase domain

PMID:19920124[6]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=entF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128569

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945184

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002021

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P11454

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10264

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10264

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945184

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000258

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0260

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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