cyaY:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
CyaY |
|---|---|
| Synonyms |
frataxin, iron-binding and oxidizing protein[1], B3807[2][1], CyaY[2][1] , ECK3801, JW3779, b3807 |
| Product description |
iron-binding frataxin homolog[2][3] Iron donor during [Fe-S] cluster assembly on IscU; iron-binding and oxidizing protein[4] |
| EC number (for enzymes) |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
Fig. 5 and Fig. 6. |
complete | |||
| GO:0016226 |
iron-sulfur cluster assembly |
IDA: Inferred from Direct Assay |
P |
In vitro iron donor to IscU in the presence of IscS and cysteine (Fig. 6). |
complete | |||
| GO:0016226 |
iron-sulfur cluster assembly |
IGI: Inferred from Genetic Interaction |
P |
Complementation of a yeast strain depleted of the endogenous frataxin protein (yfh1Delta). |
complete | |||
| GO:0008199 |
ferric iron binding |
IDA: Inferred from Direct Assay |
F |
Strong binding of Fe(+3) and weak binding of Fe(+2). |
complete | |||
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
dnaN |
MALDI(Z-score):24.136689 | ||
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Notes
CyaY is proposed to be an iron chaperone that sequesters free, redox active Fe.[9] In vitro, CyaY can bind iron and prevents the formation of hydroxyl free radicals in the presence of hydrogen peroxide (Fig. 5 of PMID:17244611).
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
Cytoplasm |
Western blot analysis of soluble and membrane fractions (Fig. 5). Visualization of an overproduced CyaY-Gfp fusion protein (Fig. 6). |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MNDSEFHRLA DQLWLTIEER LDDWDGDSDI DCEINGGVLT ITFENGSKII INRQEPLHQV WLATKQGGYH FDLKGDEWIC DRSGETFWDL LEQAATQQAG ETVSFR |
| Length |
106 |
| Mol. Wt |
12.231 kDa |
| pI |
4.1 (calculated) |
| Extinction coefficient |
28,990 - 29,240 (calc based on 1 Y, 5 W, and 2 C residues) |
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Domains/Motifs/Modification Sites
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
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| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Pohl, T et al. (2007) Effects of the deletion of the Escherichia coli frataxin homologue CyaY on the respiratory NADH:ubiquinone oxidoreductase. BMC Biochem. 8 13 PubMed
- ↑ 6.0 6.1 Layer, G et al. (2006) Iron-sulfur cluster biosynthesis: characterization of Escherichia coli CYaY as an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU. J. Biol. Chem. 281 16256-63 PubMed
- ↑ Bedekovics, T et al. (2007) Partial conservation of functions between eukaryotic frataxin and the Escherichia coli frataxin homolog CyaY. FEMS Yeast Res. 7 1276-84 PubMed
- ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Ding, H et al. (2007) Distinct iron binding property of two putative iron donors for the iron-sulfur cluster assembly: IscA and the bacterial frataxin ortholog CyaY under physiological and oxidative stress conditions. J. Biol. Chem. 282 7997-8004 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
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