cspA:Gene Product(s)

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CspA

Synonyms

major cold shock protein[1], B3556[2][1], CspA[2][1], major cold shock protein 7.4[2][1], CS7.4[2][1], F10.6[2][1], cold shock protein CspA[2][1] , ECK3543, JW3525, b3556

Product description

CspA transcriptional activator[2][3]

Cold-inducible RNA chaperone and anti-terminator; aids gene expression at low temperature; cold shock protein CS7.4; binds ssRNA and ssDNA[4]

EC number (for enzymes)


edit table

<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009409

response to cold

PMID:3553157[5]

IEP: Inferred from Expression Pattern

P

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011129

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012156

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002059

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003697

single-stranded DNA binding

PMID:8995247[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

PMID:8995247[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002059

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

PMID:3553157[5]

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

GO:0060567

negative regulation of transcription termination

PMID:10884409[7]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

PMID:3553157[5]

IEP: Inferred from Expression Pattern

P

CspA level increases after temperature downshift from 37 to 10°C.

complete

GO:0003677

DNA binding

PMID:7748932[8]

IDA: Inferred from Direct Assay

F

complete

GO:0003727

single-stranded RNA binding

PMID:8995247[6]

IDA: Inferred from Direct Assay

F

Figure 2

complete

GO:0003723

RNA binding

PMID:8995247[6]

IDA: Inferred from Direct Assay

F

Fig. 1-3

complete

GO:0003697

single-stranded DNA binding

PMID:8995247[6]

IDA: Inferred from Direct Assay

F

Fig. 5

complete

GO:0060567

negative regulation of transcription termination

PMID:10884409[7]

IDA: Inferred from Direct Assay

P

Fig. 1C,D

complete

GO:0001072

RNA binding transcription antitermination factor activity

PMID:10884409[7]

IDA: Inferred from Direct Assay

F

replaces obsoleteGO:0030401

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpoC

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rpsI

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

nfnB

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rplC

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rpsD

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rplA

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rpsC

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rpoB

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

phoB

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rplQ

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rpsG

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rplO

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

rplB

PMID:16606699[9]

Experiment(s):EBI-1146120

Protein

accA

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

cca

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

deaD

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

ybhF

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

ycbY

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

rluC

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

rluB

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

dnaN

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

dsbG

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

rplA

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

rplD

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

rpsD

PMID:15690043[10]

Experiment(s):EBI-879694

Protein

rplC

PMID:15690043[10]

Experiment(s):EBI-879694, EBI-884189

Protein

atpG

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

cspG

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

hfq

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

hupA

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

hupB

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rplL

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rplR

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rplS

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rpoA

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rpsC

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rpsE

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rpsG

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rpsJ

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

rpsN

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

ydaY

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

ygcG

PMID:15690043[10]

Experiment(s):EBI-884189

Protein

atpG

PMID:19402753[11]

LCMS(ID Probability):99.0

Protein

rplR

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

rlmL

PMID:19402753[11]

MALDI(Z-score):25.000790

Protein

rplS

PMID:19402753[11]

LCMS(ID Probability):99.0 MALDI(Z-score):5.581639

Protein

rpsJ

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

hupB

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

cspG

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

hfq

PMID:19402753[11]

LCMS(ID Probability):99.0

Protein

rluB

PMID:19402753[11]

MALDI(Z-score):36.206042

Protein

ygcG

PMID:19402753[11]

LCMS(ID Probability):99.0

Protein

rplL

PMID:19402753[11]

LCMS(ID Probability):99.6

edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm


edit table

<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK VSFTIESGAK
GPAAGNVTSL
Length

70

Mol. Wt

7.402 kDa

pI

5.9 (calculated)

Extinction coefficient

6,990 (calc based on 1 Y, 1 W, and 0 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A9X9

Domain

4..70

PF00313 'Cold-shock' DNA-binding domain

PMID:19920124[12]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cspA taxon=562,83333 </beststructure>

Models

View models at:

edit table
</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131427

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948070

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011616

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A9X9

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10166

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10166

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948070

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000162

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0164

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Jones, PG et al. (1987) Induction of proteins in response to low temperature in Escherichia coli. J. Bacteriol. 169 2092-5 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 Jiang, W et al. (1997) CspA, the major cold-shock protein of Escherichia coli, is an RNA chaperone. J. Biol. Chem. 272 196-202 PubMed
  7. ↑ 7.0 7.1 7.2 Bae, W et al. (2000) Escherichia coli CspA-family RNA chaperones are transcription antiterminators. Proc. Natl. Acad. Sci. U.S.A. 97 7784-9 PubMed
  8. ↑ Brandi, A et al. (1994) Interaction of the main cold shock protein CS7.4 (CspA) of Escherichia coli with the promoter region of hns. Biochimie 76 1090-8 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ 10.00 10.01 10.02 10.03 10.04 10.05 10.06 10.07 10.08 10.09 10.10 10.11 10.12 10.13 10.14 10.15 10.16 10.17 10.18 10.19 10.20 10.21 10.22 10.23 10.24 10.25 10.26 10.27 10.28 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  11. ↑ 11.00 11.01 11.02 11.03 11.04 11.05 11.06 11.07 11.08 11.09 11.10 11.11 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  12. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

[back to top]


Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=cspA:Gene_Product(s)&oldid=1495339"