clpB:Gene Product(s)

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ClpB

Synonyms

protein disaggregation chaperone[1], B2592[2][1], HtpM[2][1], ClpB[2][1], F84.1[2][1], F68.5[2][1], E89[2][1] , ECK2590, htpM, JW2573, b2592

Product description

ClpB chaperone[2][3]

ATP-dependent protease and chaperone; protein disaggregation chaperone; role in de novo protein folding under mild stress conditions[4]

EC number (for enzymes)


edit table

<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004176

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001270

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003959

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013093

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018368

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0019538

protein metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004176

P

Seeded from EcoCyc (v14.0)

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsB

PMID:15690043[6]

Experiment(s):EBI-884811

Protein

tufA

PMID:15690043[6]

Experiment(s):EBI-884811

Protein

ydeP

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

ccmB

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rnz

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

glcB

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

lasT

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

ptsN

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rplC

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rplM

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rplU

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rplV

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rpmC

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rpsG

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rpsH

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rpsJ

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

rpsN

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

sapC

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

thiG

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

yagW

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

ygaU

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

yhfT

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

gfcB

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

evgS

PMID:15690043[6]

Experiment(s):EBI-891673

Protein

putA

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

uidC

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

lacZ

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

ptsP

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

yjbH

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

alaS

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

ulaF

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

phoB

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

rplB

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

sdhA

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

nadE

PMID:16606699[7]

Experiment(s):EBI-1143404

Protein

tufB

PMID:19402753[8]

MALDI(Z-score):19.933263

Protein

tufB

PMID:19402753[8]

MALDI(Z-score):19.933263

Small Molecule

AppppA (5',5'''-P1,P4-diadenosine tetraphosphate)

PMID:8468292[9]

Photo-crosslinking with radioactively labelled azido-AppppA followed by 1-D and 2-D PAGE.

edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm


edit table

<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MRLDRLTNKF QLALADAQSL ALGHDNQFIE PLHLMSALLN QEGGSVSPLL TSAGINAGQL
RTDINQALNR LPQVEGTGGD VQPSQDLVRV LNLCDKLAQK RGDNFISSEL FVLAALESRG
TLADILKAAG ATTANITQAI EQMRGGESVN DQGAEDQRQA LKKYTIDLTE RAEQGKLDPV
IGRDEEIRRT IQVLQRRTKN NPVLIGEPGV GKTAIVEGLA QRIINGEVPE GLKGRRVLAL
DMGALVAGAK YRGEFEERLK GVLNDLAKQE GNVILFIDEL HTMVGAGKAD GAMDAGNMLK
PALARGELHC VGATTLDEYR QYIEKDAALE RRFQKVFVAE PSVEDTIAIL RGLKERYELH
HHVQITDPAI VAAATLSHRY IADRQLPDKA IDLIDEAASS IRMQIDSKPE ELDRLDRRII
QLKLEQQALM KESDEASKKR LDMLNEELSD KERQYSELEE EWKAEKASLS GTQTIKAELE
QAKIAIEQAR RVGDLARMSE LQYGKIPELE KQLEAATQLE GKTMRLLRNK VTDAEIAEVL
ARWTGIPVSR MMESEREKLL RMEQELHHRV IGQNEAVDAV SNAIRRSRAG LADPNRPIGS
FLFLGPTGVG KTELCKALAN FMFDSDEAMV RIDMSEFMEK HSVSRLVGAP PGYVGYEEGG
YLTEAVRRRP YSVILLDEVE KAHPDVFNIL LQVLDDGRLT DGQGRTVDFR NTVVIMTSNL
GSDLIQERFG ELDYAHMKEL VLGVVSHNFR PEFINRIDEV VVFHPLGEQH IASIAQIQLK
RLYKRLEERG YEIHISDEAL KLLSENGYDP VYGARPLKRA IQQQIENPLA QQILSGELVP
GKVIRLEVNE DRIVAVQ
Length

857

Mol. Wt

95.587 kDa

pI

5.3 (calculated)

Extinction coefficient

36,330 - 36,705 (calc based on 17 Y, 2 W, and 3 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

202..341

PF00004 ATPase family associated with various cellular activities (AAA)

PMID:19920124[10]

Domain

17..69

PF02861 Clp amino terminal domain

PMID:19920124[10]

Domain

94..145

PF02861 Clp amino terminal domain

PMID:19920124[10]

Domain

596..760

PF07724 AAA domain (Cdc48 subfamily)

PMID:19920124[10]

Domain

766..854

PF10431 C-terminal, D2-small domain, of ClpB protein

PMID:19920124[10]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=clpB taxon=562,83333 </beststructure>

Models

View models at:

edit table
</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130513

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947077

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008527

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P63284

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10157

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10157

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947077

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000153

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0155

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ 8.0 8.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Fuge, EK & Farr, SB (1993) AppppA-binding protein E89 is the Escherichia coli heat shock protein ClpB. J. Bacteriol. 175 2321-6 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

[back to top]


Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=clpB:Gene_Product(s)&oldid=1494457"