chaA:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
ChaA |
|---|---|
| Synonyms |
calcium/sodium:proton antiporter[1], B1216[2][1], ChaA[2][1] , ECK1210, JW1207, b1216 |
| Product description |
ChaA calcium CaCA transporter[2][3] Ca2+/H+ and K+/H+ antiporter; extrudes potassium ions, and also sodium ions at alkaline pH; regulated by osmolarity and pH[4] |
| EC number (for enzymes) |
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| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005509 |
calcium ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006813 |
potassium ion transport |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0006814 |
sodium ion transport |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0006814 |
sodium ion transport |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006816 |
calcium ion transport |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006883 |
cellular sodium ion homeostasis |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0015385 |
sodium:hydrogen antiporter activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0015386 |
potassium:hydrogen antiporter activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0015491 |
cation:cation antiporter activity |
IGI: Inferred from Genetic Interaction |
F |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0015992 |
proton transport |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0016020 |
membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030007 |
cellular potassium ion homeostasis |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0005887 |
integral to plasma membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0051481 |
reduction of cytosolic calcium ion concentration |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0055085 |
transmembrane transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055085 |
transmembrane transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
plasma membrane |
C-terminus localized to the periplasm with 11 predicted transmembrane domains |
Daley et al. (2005) [11] |
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|
plasma membrane |
From EcoCyc[3] |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSNAQEAVKT RHKETSLIFP VLALVVLFLW GSSQTLPVVI AINLLALIGI LSSAFSVVRH ADVLAHRLGE PYGSLILSLS VVILEVSLIS ALMATGDAAP TLMRDTLYSI IMIVTGGLVG FSLLLGGRKF ATQYMNLFGI KQYLIALFPL AIIVLVFPMA LPAANFSTGQ ALLVALISAA MYGVFLLIQT KTHQSLFVYE HEDDSDDDDP HHGKPSAHSS LWHAIWLIIH LIAVIAVTKM NASSLETLLD SMNAPVAFTG FLVALLILSP EGLGALKAVL NNQVQRAMNL FFGSVLATIS LTVPVVTLIA FMTGNELQFA LGAPEMVVMV ASLVLCHISF STGRTNVLNG AAHLALFAAY LMTIFA |
| Length |
366 |
| Mol. Wt |
39.169 kDa |
| pI |
6.7 (calculated) |
| Extinction coefficient |
26,930 - 27,055 (calc based on 7 Y, 3 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Radchenko, MV et al. (2006) Potassium/proton antiport system of Escherichia coli. J. Biol. Chem. 281 19822-9 PubMed
- ↑ 6.0 6.1 Trchounian, A & Kobayashi, H (1999) Fermenting Escherichia coli is able to grow in media of high osmolarity, but is sensitive to the presence of sodium ion. Curr. Microbiol. 39 109-14 PubMed
- ↑ 7.0 7.1 7.2 7.3 Ivey, DM et al. (1993) Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene. J. Biol. Chem. 268 11296-303 PubMed
- ↑ 8.0 8.1 Ohyama, T et al. (1994) Physiological role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli. J. Bacteriol. 176 4311-5 PubMed
- ↑ Shijuku, T et al. (2002) Expression of chaA, a sodium ion extrusion system of Escherichia coli, is regulated by osmolarity and pH. Biochim. Biophys. Acta 1556 142-8 PubMed
- ↑ Hsieh, JM et al. (2010) Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini. Protein Sci. 19 868-80 PubMed
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0005509 ! calcium ion binding
- GO:0005886 ! plasma membrane
- GO:0006813 ! potassium ion transport
- GO:0006814 ! sodium ion transport
- GO:0006816 ! calcium ion transport
- GO:0006883 ! cellular sodium ion homeostasis
- GO:0015385 ! sodium:proton antiporter activity
- GO:0015386 ! potassium:proton antiporter activity
- GO:0015491 ! cation:cation antiporter activity
- GO:0015992 ! proton transport
- GO:0016020 ! membrane
- GO:0016021 ! integral component of membrane
- GO:0030007 ! cellular potassium ion homeostasis
- GO:0005887 ! integral component of plasma membrane
- GO:0051481 ! negative regulation of cytosolic calcium ion concentration
- GO:0055085 ! transmembrane transport
- TMHMM Prediction
- Proteins
