PMID:16687400
| Citation |
Radchenko, MV, Tanaka, K, Waditee, R, Oshimi, S, Matsuzaki, Y, Fukuhara, M, Kobayashi, H, Takabe, T and Nakamura, T (2006) Potassium/proton antiport system of Escherichia coli. J. Biol. Chem. 281:19822-9 |
|---|---|
| Abstract |
The intracellular level of potassium (K(+)) in Escherichia coli is regulated through multiple K(+) transport systems. Recent data indicate that not all K(+) extrusion system(s) have been identified (15). Here we report that the E. coli Na(+) (Ca(2+))/H(+) antiporter ChaA functions as a K(+) extrusion system. Cells expressing ChaA mediated K(+) efflux against a K(+) concentration gradient. E. coli strains lacking the chaA gene were unable to extrude K(+) under conditions in which wild-type cells extruded K(+). The K(+)/H(+) antiporter activity of ChaA was detected by using inverted membrane vesicles produced using a French press. Physiological growth studies indicated that E. coli uses ChaA to discard excessive K(+), which is toxic for these cells. These results suggest that ChaA K(+)/H(+) antiporter activity enables E. coli to adapt to K(+) salinity stress and to maintain K(+) homeostasis. |
| Links |
PubMed Online version:10.1074/jbc.M600333200 |
| Keywords |
Base Sequence; Biological Transport; DNA Primers; Escherichia coli/metabolism; Ethanolamines/pharmacology; Potassium/metabolism; Potassium Chloride/metabolism; Potassium-Hydrogen Antiporters/genetics; Potassium-Hydrogen Antiporters/metabolism |
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