add:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Add |
|---|---|
| Synonyms |
adenosine deaminase[1], B1623[2][1], Add[2][1] , ECK1618, JW1615, b1623 |
| Product description |
Adenosine deaminase; mutants affect growth on deoxyadenosine in purA, B mutants[3] |
| EC number (for enzymes) | |
| edit table |
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Notes
Adenosine deaminase is the enzyme that catalyzes the irreversible deamination of adenosine or deoxyadenosine to inosine or deoxyinosine. The active site of ADA contains residues of a Histidine, 2 Aspartic acids, one Glutamic acid, and a zinc atom. The overall mechanism involves a hydroxyl group addition to C6 of adenosine, which forms the tetrahedral transition state intermediate. Then, the ammonia elimination forms inosine. Zinc acts as a powerful electrophile, which allows a water molecule to attack C6. Glutamic acid interacts with N1 and donates a proton, reducing the N1=C6 double bond character, allowing the hydroxyl nucleophilic attack. A Histidine residue and the carboxylic group of aspartic acid stabilizes and orients the zinc-bound water molecule. The ammonia elimination pathway is not quite understood but is the final step in the mechanism of adenosine deaminase.
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005737 |
cytoplasm |
C |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
| GO:0004000 |
adenosine deaminase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0004000 |
adenosine deaminase activity |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0015950 |
purine nucleotide interconversion |
IGI: Inferred from Genetic Interaction |
EcoliWiki:guaA
|
P |
complete | |||
| GO:0004000 |
adenosine deaminase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009168 |
purine ribonucleoside monophosphate biosynthetic process |
IGI: Inferred from Genetic Interaction |
EcoliWiki:guaA
|
P |
complete | |||
| GO:0004000 |
adenosine deaminase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005488 |
binding |
ISS: Inferred from Sequence or Structural Similarity |
F |
CHEBI:27834 (Deoxycoformycin mechanistically inhibits Add). |
complete | |||
| GO:0006154 |
adenosine catabolic process |
IGI: Inferred from Genetic Interaction |
EcoliWiki:guaA
|
P |
complete | |||
| GO:0004000 |
adenosine deaminase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004000 |
adenosine deaminase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0032261 |
purine nucleotide salvage |
IGI: Inferred from Genetic Interaction |
EcoliWiki:guaA
|
P |
complete | |||
| GO:0006154 |
adenosine catabolic process |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0043103 |
hypoxanthine salvage |
IGI: Inferred from Genetic Interaction |
EcoliWiki:guaA
|
P |
complete | |||
| GO:0009117 |
nucleotide metabolic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046101 |
hypoxanthine biosynthetic process |
IGI: Inferred from Genetic Interaction |
EcoliWiki:guaA
|
P |
complete | |||
| GO:0009168 |
purine ribonucleoside monophosphate biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IC: Inferred by Curator |
C |
Missing: with/from | ||||
| GO:0009168 |
purine ribonucleoside monophosphate biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009168 |
purine ribonucleoside monophosphate biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009168 |
purine ribonucleoside monophosphate biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009168 |
purine ribonucleoside monophosphate biosynthetic process |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0015950 |
purine nucleotide interconversion |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0032261 |
purine nucleotide salvage |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0043103 |
hypoxanthine salvage |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0006974 |
response to DNA damage stimulus |
IEP: Inferred from Expression Pattern |
P |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
rpsM |
Experiment(s):EBI-1140315 | ||
|
Protein |
arpA |
Experiment(s):EBI-1140315 | ||
|
Protein |
rplT |
Experiment(s):EBI-1140315 | ||
|
Protein |
insC1 |
Experiment(s):EBI-1140315 | ||
|
Protein |
ligA |
Experiment(s):EBI-1140315 | ||
|
Protein |
gcd |
Experiment(s):EBI-1140315 | ||
|
Small Molecule |
CHEBI:27834 (Deoxycoformycin) |
Inhibitor of Add activity |
| |
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MIDTTLPLTD IHRHLDGNIR PQTILELGRQ YNISLPAQSL ETLIPHVQVI ANEPDLVSFL TKLDWGVKVL ASLDACRRVA FENIEDAARH GLHYVELRFS PGYMAMAHQL PVAGVVEAVI DGVREGCRTF GVQAKLIGIM SRTFGEAACQ QELEAFLAHR DQITALDLAG DELGFPGSLF LSHFNRARDA GWHITVHAGE AAGPESIWQA IRELGAERIG HGVKAIEDRA LMDFLAEQQI GIESCLTSNI QTSTVAELAA HPLKTFLEHG IRASINTDDP GVQGVDIIHE YTVAAPAAGL SREQIRQAQI NGLEMAFLSA EEKRALREKV AAK |
| Length |
333 |
| Mol. Wt |
36.397 kDa |
| pI |
5.4 (calculated) |
| Extinction coefficient |
22,460 - 22,960 (calc based on 4 Y, 3 W, and 4 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Remy, CN & Love, SH (1968) Induction of adenosine deaminase in Escherichia coli. J. Bacteriol. 96 76-85 PubMed
- ↑ Jochimsen, B et al. (1975) Location on the chromosome of Escherichia coli of genes governing purine metabolism. Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt). Mol. Gen. Genet. 143 85-91 PubMed
- ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 Chang, ZY et al. (1991) Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function. Biochemistry 30 2273-80 PubMed
- ↑ Frick, L et al. (1986) Transition-state stabilization by adenosine deaminase: structural studies of its inhibitory complex with deoxycoformycin. Biochemistry 25 1616-21 PubMed
- ↑ 8.0 8.1 Wolfenden, R et al. (1967) Substrate binding by adenosine deaminase. Specificity, pH dependence, and competition by mercurials. J. Biol. Chem. 242 977-83 PubMed
- ↑ Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed
- ↑ 10.0 10.1 10.2 10.3 10.4 10.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0005737 ! cytoplasm
- GO:0004000 ! adenosine deaminase activity
- GO:0015950 ! purine nucleotide interconversion
- GO:0009168 ! purine ribonucleoside monophosphate biosynthetic process
- GO:0005488 ! binding
- GO:0006154 ! adenosine catabolic process
- GO:0032261 ! purine nucleotide salvage
- GO:0043103 ! hypoxanthine salvage
- GO:0009117 ! nucleotide metabolic process
- GO:0046101 ! hypoxanthine biosynthetic process
- GO:0006974 ! cellular response to DNA damage stimulus
- Proteins
- RefGenome Annotated Gene
