msbA:Gene Product(s)
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
MsbA |
|---|---|
| Synonyms |
fused lipid transporter subunits of ABC superfamily: membrane component[1], ATP-binding component[1], B0914[2][1], MsbA[2][1] |
| Product description |
ATP-binding transport protein[2][3] Lipid exporter to the outer membrane; exports LPS, phospholipids, and lipid A; drug export and resistance; ABC transporter flippase; biogenesis of outer membrane; lipid-activated ATPase[4] |
| EC number (for enzymes) |
|
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005319 |
lipid transporter activity |
IMP: Inferred from Mutant Phenotype |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc [9] |
complete | |||
| GO:0006869 |
lipid transport |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0015437 |
lipopolysaccharide-transporting ATPase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc [9] |
complete | |||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [9] |
complete | |||
| GO:0005319 |
lipid transporter activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005524 |
ATP binding |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001140 |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |
| GO:0006810 |
transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [9] |
complete | |||
| GO:0006869 |
lipid transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0009276 |
Gram-negative-bacterium-type cell wall |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc [9] |
complete | |||
| GO:0016020 |
membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001140 |
C |
Seeded from EcoCyc [9] |
complete | ||
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [9] |
complete | |||
| GO:0016887 |
ATPase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0017111 |
nucleoside-triphosphatase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [9] |
complete | |||
| GO:0042626 |
ATPase activity, coupled to transmembrane movement of substances |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [9] |
complete | |||
| GO:0005739 |
mitochondrion |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc [9] |
complete | |||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc [9] |
complete | ||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0005319 |
lipid transporter activity |
IMP: Inferred from Mutant Phenotype |
F |
Seeded from EcoCyc |
complete | |||
| GO:0005524 |
ATP binding |
GO_REF:0000002 |
IDA: Inferred from Direct Assay |
InterPro:IPR001140 |
F |
Seeded from EcoCyc |
complete | |
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc |
complete | |||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc |
complete | ||
| GO:0006810 |
transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc |
complete | |||
| GO:0006869 |
lipid transport |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc |
complete | |||
| GO:0009276 |
Gram-negative-bacterium-type cell wall |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc |
complete | |||
| GO:0015437 |
lipopolysaccharide-transporting ATPase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc |
complete | |||
| GO:0016020 |
membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc |
complete | |||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001140 |
C |
Seeded from EcoCyc |
complete | ||
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc |
complete | |||
| GO:0017111 |
nucleoside-triphosphatase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0042626 |
ATPase activity, coupled to transmembrane movement of substances |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Notes |
|---|---|---|---|
|
plasma membrane |
C-terminus localized in the cytoplasm with 6 predicted transmembrane domains |
Daley et al. (2005) [11] |
|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MHNDKDLSTW QTFRRLWPTI APFKAGLIVA GVALILNAAS DTFMLSLLKP LLDDGFGKTD RSVLVWMPLV VIGLMILRGI TSYVSSYCIS WVSGKVVMTM RRRLFGHMMG MPVSFFDKQS TGTLLSRITY DSEQVASSSS GALITVVREG ASIIGLFIMM FYYSWQLSII LIVLAPIVSI AIRVVSKRFR NISKNMQNTM GQVTTSAEQM LKGHKEVLIF GGQEVETKRF DKVSNRMRLQ GMKMVSASSI SDPIIQLIAS LALAFVLYAA SFPSVMDSLT AGTITVVFSS MIALMRPLKS LTNVNAQFQR GMAACQTLFT ILDSEQEKDE GKRVIERATG DVEFRNVTFT YPGRDVPALR NINLKIPAGK TVALVGRSGS GKSTIASLIT RFYDIDEGEI LMDGHDLREY TLASLRNQVA LVSQNVHLFN DTVANNIAYA RTEQYSREQI EEAARMAYAM DFINKMDNGL DTVIGENGVL LSGGQRQRIA IARALLRDSP ILILDEATSA LDTESERAIQ AALDELQKNR TSLVIAHRLS TIEKADEIVV VEDGVIVERG THNDLLEHRG VYAQLHKMQF GQ |
|---|---|
| Length |
582 |
| Mol. Wt |
64.462 kDa |
| pI |
8.8 (calculated) |
| Extinction coefficient |
46,870 - 47,120 (calc based on 13 Y, 5 W, and 2 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/>
|
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 Doerrler, WT et al. (2001) An Escherichia coli mutant defective in lipid export. J. Biol. Chem. 276 11461-4 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 7.0 7.1 Polissi, A & Georgopoulos, C (1996) Mutational analysis and properties of the msbA gene of Escherichia coli, coding for an essential ABC family transporter. Mol. Microbiol. 20 1221-33 PubMed
- ↑ 8.0 8.1 8.2 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 10.0 10.1 10.2 10.3 Doerrler, WT & Raetz, CR (2002) ATPase activity of the MsbA lipid flippase of Escherichia coli. J. Biol. Chem. 277 36697-705 PubMed
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ 12.0 12.1 12.2 12.3 12.4 Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed
Categories
- GO:0005319 ! lipid transporter activity
- GO:0005524 ! ATP binding
- GO:0005624 ! obsolete membrane fraction
- GO:0006869 ! lipid transport
- GO:0015437 ! lipopolysaccharide floppase activity
- GO:0016887 ! ATPase activity
- GO:0000166 ! nucleotide binding
- GO:0006810 ! transport
- GO:0009276 ! Gram-negative-bacterium-type cell wall
- GO:0016020 ! membrane
- GO:0016021 ! integral component of membrane
- GO:0016787 ! hydrolase activity
- GO:0017111 ! nucleoside-triphosphatase activity
- GO:0042626 ! ATPase-coupled transmembrane transporter activity
- GO:0005739 ! mitochondrion
- GO:0005886 ! plasma membrane
- TMHMM Prediction
- Proteins