ada:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Ada |
|---|---|
| Synonyms |
fused DNA-binding transcriptional dual regulator[1], O6-methylguanine-DNA methyltransferase[1], B2213[2][1], Ada[2][1] |
| Product description | |
| EC number (for enzymes) | |
| edit table |
Notes
Adenosine deaminase is the enzyme that catalyzes the irreversible deamination of adenosine or deoxyadenosine to inosine or deoxyinosine. The active site of ADA contains residues of a Histidine, 2 Aspartic acids, one Glutamic acid, and a zinc atom. The overall mechanism involves a hydroxyl group addition to C6 of adenosine, which forms the tetrahedral transition state intermediate. Then, the ammonia elimination forms inosine. Zinc acts as a powerful electrophile, which allows a water molecule to attack C6. Glutamic acid interacts with N1 and donates a proton, reducing the N1=C6 double bond character, allowing the hydroxyl nucleophilic attack. A Histidine residue and the carboxylic group of aspartic acid stabilizes and orients the zinc-bound water molecule. The ammonia elimination pathway is not quite understood but is the final step in the mechanism of adenosine deaminase.
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005622 |
intracellular |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0003908 |
methylated-DNA-(protein)-cysteine S-methyltransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0003677 |
DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0003700 |
transcription factor activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0016740 |
transferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0043565 |
sequence-specific DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006281 |
DNA repair |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001497 |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |
| GO:0006350 |
transcription |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006355 |
regulation of transcription, DNA-dependent |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006974 |
response to DNA damage stimulus |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0008168 |
methyltransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0008270 |
zinc ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005737 |
cytoplasm |
C |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0016563 |
transcription activator activity |
F |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0016564 |
transcription repressor activity |
F |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
rplB |
Experiment(s):EBI-1142195 | ||
|
Protein |
rpsC |
Experiment(s):EBI-1142195 | ||
|
Protein |
moeB |
Experiment(s):EBI-1142195 | ||
|
Protein |
fur |
Experiment(s):EBI-1142195 | ||
|
Protein |
rpsG |
Experiment(s):EBI-1142195 | ||
|
Protein |
ybiA |
Experiment(s):EBI-1142195 | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Notes |
|---|---|---|---|
|
membrane |
From EcoCyc[3] | ||
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL RESLAKGESV TTSILNAGFP DSSSYYRKAD ETLGMTAKQF RHGGENLAVR YALADCELGR CLVAESERGI CAILLGDDDA TLISELQQMF PAADNAPADL MFQQHVREVI ASLNQRDTPL TLPLDIRGTA FQQQVWQALR TIPCGETVSY QQLANAIGKP KAVRAVASAC AANKLAIIIP CHRVVRGDGT LSGYRWGVSR KAQLLRREAE NEER |
|---|---|
| Mol. Wt |
39.32 kDa (calc) [2] |
| pI |
8.69[2] |
| Extinction coefficient |
36440 - 37940 (calc based on 6 Y, 5 W, and 12 C residues) |
| edit table |
Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
| Type | Residues | Description | Notes |
|---|---|---|---|
| edit table |
Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.
| Structures |
<beststructure>ada 562</beststructure> |
|---|---|
| Models |
View models at: |
| edit table |
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
Categories
- GO:0005622 ! intracellular
- GO:0003908 ! methylated-DNA-(protein)-cysteine S-methyltransferase activity
- GO:0003677 ! DNA binding
- GO:0003700 ! DNA-binding transcription factor activity
- GO:0003824 ! catalytic activity
- GO:0046872 ! metal ion binding
- GO:0016740 ! transferase activity
- GO:0043565 ! sequence-specific DNA binding
- GO:0006281 ! DNA repair
- GO:0006350 ! transcription
- GO:0006355 ! regulation of transcription, DNA-templated
- GO:0006974 ! cellular response to DNA damage stimulus
- GO:0008168 ! methyltransferase activity
- GO:0008270 ! zinc ion binding
- GO:0005737 ! cytoplasm
- GO:0016563 ! obsolete transcription activator activity
- GO:0016564 ! obsolete transcription repressor activity
- Proteins
- GO:0016563 ! transcription activator activity
- GO:0016564 ! transcription repressor activity
- Location:membrane