SOS Response

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General Description

A set of reversible checkpoints induced upon DNA damage, or regions of single stranded DNA.

Components

LexA
RecA
SulA (a.k.a. SfiA)

Function

To postpone Septation until DNA is repaired.

Regulation

RecA bound to single-stranded DNA leads to its activation. This activated form of RecA, or RecA* causes autocleavage of the LexA repressor^[1]. It was later discovered that the mechanism of LexA cleavage is autodigestion catalyzed by RecA*^[2].

Cell Biology

The expression of multiple genes is upregulated upon induction of the SOS response. The LexA repressor control the expression of:

sulA^[3]
ftsK^[4]
uvrA^[5]
uvrB^[6]
Peterson et al. (1988) finds that the derepression of other specific genes promote DNA repair and mutagenesis^[7].

Experimental Resources

Several antibiotics induce the SOS response

mitomycin C (working concentration-0.1 µg/ml)^[4]

Comparison with other organisms

Notes

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References

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↑ Little, JW () Control of the SOS regulatory system by the level of RecA protease. Biochimie 64 585-9 PubMed
↑ Little, JW (1984) Autodigestion of lexA and phage lambda repressors. Proc. Natl. Acad. Sci. U.S.A. 81 1375-9 PubMed
↑ Mizusawa, S et al. (1983) Transcription of the sulA gene and repression by LexA. J. Mol. Biol. 171 337-43 PubMed
↑ ^4.0 ^4.1 Wang, L & Lutkenhaus, J (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol. Microbiol. 29 731-40 PubMed
↑ Sancar, A et al. (1982) LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature 298 96-8 PubMed
↑ Sancar, GB et al. (1982) The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters. Cell 28 523-30 PubMed
↑ Peterson, KR et al. (1988) Derepression of specific genes promotes DNA repair and mutagenesis in Escherichia coli. J. Bacteriol. 170 1-4 PubMed

[PMID:6814506-1] Little, JW () Control of the SOS regulatory system by the level of RecA protease. Biochimie 64 585-9 PubMed

[PMID:6231641-2] Little, JW (1984) Autodigestion of lexA and phage lambda repressors. Proc. Natl. Acad. Sci. U.S.A. 81 1375-9 PubMed

[PMID:6317868-3] Mizusawa, S et al. (1983) Transcription of the sulA gene and repression by LexA. J. Mol. Biol. 171 337-43 PubMed

[PMID:9723913-4] 4.0 ^4.1 Wang, L & Lutkenhaus, J (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol. Microbiol. 29 731-40 PubMed

[PMID:6283374-5] Sancar, A et al. (1982) LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature 298 96-8 PubMed

[PMID:6280873-6] Sancar, GB et al. (1982) The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters. Cell 28 523-30 PubMed

[PMID:3275605-7] Peterson, KR et al. (1988) Derepression of specific genes promotes DNA repair and mutagenesis in Escherichia coli. J. Bacteriol. 170 1-4 PubMed

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